Bovine Foot and Mouth Disease Type A Polypeptide Vaccine

A technology of bovine foot-and-mouth disease and polypeptide vaccines, applied in the field of medicine, can solve the problems that affect the use of new vaccines, cannot effectively protect animals, and have poor effects

Active Publication Date: 2019-03-19
CHINA ANIMAL HUSBANDRY IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In terms of research on new vaccines for foot-and-mouth disease, there have been reports on genetically engineered subunit vaccines, foot-and-mouth disease virus vector vaccines, and genetically engineered vaccines for foot-and-mouth disease. use of vaccines
In addition, these vaccines are often less effective against currently circulating strains that have mutated and cannot effectively protect animals

Method used

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  • Bovine Foot and Mouth Disease Type A Polypeptide Vaccine
  • Bovine Foot and Mouth Disease Type A Polypeptide Vaccine
  • Bovine Foot and Mouth Disease Type A Polypeptide Vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, the solid-phase synthesis of bovine foot-and-mouth disease type A polypeptide vaccine polypeptide

[0075] The present invention studies the variation of the main antigenic sites of the FMD by determining the sequence of the newly popular strains of the domestic FMD and combining the sequences of the FMD vaccine strains, and counts the variation frequency of the main mutated amino acid sites, and at the same time combines computer-aided FMD Analysis and prediction of antigenic sites, chemical synthesis of possible antigenic site peptides, that is, according to the statistical variation frequency of variable sites, different amino acids are used at these sites to obtain a multiplicity covering all current possible variable sites A candidate polypeptide antigen. Furthermore, these candidate polypeptide antigens were screened through a large number of animal experiments, and the polypeptide antigens capable of inducing an immune response in animals with a hi...

Embodiment 2

[0119] Embodiment 2, the preparation of bovine foot-and-mouth disease type A polypeptide vaccine

[0120] 1.1 Preparation of antigen aqueous phase

[0121] Weigh the synthetic peptide antigens shown in Polypeptide 1, Polypeptide 2, Polypeptide 3 or Polypeptide 4 prepared according to Example 1, mix them according to the molar ratio of the antigens at 1:1:1:1, and then use sterile water for injection to dissolve the synthetic peptide antigens The total antigen concentration was diluted to 50 μg / ml. The resulting antigen solution was filtered through a filter with a pore size of 0.2 μm and sterilized.

[0122] 1.2 Preparation of oil phase adjuvant

[0123] Sterilize the oil phase adjuvant at 50V at 121°C for 30min, and set aside.

[0124] 1.3 Emulsion of synthetic peptide vaccine

[0125] Clean the IKA emulsification equipment with 2000ml of sterilized distilled water for 3 times, then put the oil phase into the emulsification tank at 20-28°C according to the volume ratio ...

Embodiment 3

[0127] Embodiment 3, the effect test of bovine foot-and-mouth disease type A polypeptide vaccine

[0128] 1. Materials and methods

[0129] 1.1 Materials

[0130] 1.1.1 Synthetic peptide vaccines

[0131] According to Example 1, polypeptide antigens with sequences such as Polypeptide 1, Polypeptide 2, Polypeptide 3 or Polypeptide 4 were prepared, and then according to Example 2, the A-type foot-and-mouth disease synthetic peptides containing Polypeptide 1, Polypeptide 2, Polypeptide 3 and Polypeptide 4 mixed components were prepared. Vaccines, the corresponding batch numbers are: ZM6266201, ZM6266202, ZM6266203. Simultaneously, according to the method of Example 2, polypeptide 1, polypeptide 2, polypeptide 3 or polypeptide 4 are respectively one-component foot-and-mouth disease type A polypeptide vaccines that are antigens, and the corresponding batch numbers are: ZMDKY001, ZMDKY002, ZMDKY003, ZMDKY004.

[0132] 1.1.2 Test animals

[0133] 122 6-month-old healthy yellow ...

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Abstract

The invention discloses an antigen polypeptide, a polypeptide composition and a vaccine for preparing a bovine foot and mouth disease A-type polypeptide vaccine. The polypeptide composition comprises a polypeptide obtained by connecting the polypeptide fragment shown in sequence 1 with the polypeptide fragment shown in sequence 5. The vaccine provided by the invention comprises the polypeptide composition. The bovine foot and mouth disease A type polypeptide vaccine provided by the invention has good immune potency, so that the problems of fever, red and swollen caused by the traditional vaccine cannot be triggered. The vaccine provided by the invention can effectively response the antigenic variation of the current foot and mouth disease virus, is free of biological safety, easy for large-scale synthesize and has a good application future. The invention additionally discloses a preparation method of the polypeptide and the polypeptide vaccine and a pharmaceutical application thereof.

Description

technical field [0001] The invention relates to the technical field of medicine, and in particular, the invention relates to a bovine type A foot-and-mouth disease polypeptide vaccine and a preparation method thereof. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, highly contagious, febrile infectious disease of cloven-hoofed animals, which is widely distributed in the world. Foot-and-mouth disease is highly contagious and spreads rapidly. After infecting pigs, cattle, sheep and other livestock, it will lead to the death of young animals and a sharp decline in the production capacity of adult animals. Therefore, it seriously endangers the development of animal husbandry and the production and supply of meat and animal products. At present, foot-and-mouth disease has greatly blocked and restricted the market circulation and international trade of animals and animal products, causing huge economic losses to animal husbandry production in endemic countr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K39/135A61P31/14
CPCA61K39/135C07K14/005C12N2770/32134
Inventor 肖进宋芳齐鹏巴利民栗利芳王楠董春娜张蕾马爱荣张爱民何平安
Owner CHINA ANIMAL HUSBANDRY IND
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