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PCR detection method for poppy

A detection method, poppy technology, which is applied in the field of poppy detection, can solve the problems of narrow identification range, difficulty in accurate identification of poppy, and long identification work cycle

Inactive Publication Date: 2015-06-03
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional identification methods of opium poppy plants are mainly based on their morphological or anatomical characteristics, and because the structures of poppy plants of closely related species in taxonomy are very similar, this brings some difficulties to the accurate identification of opium poppy species, such as identifying The working cycle is long, the scope of identification is narrow, etc., and it is easy to make mistakes

Method used

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  • PCR detection method for poppy
  • PCR detection method for poppy
  • PCR detection method for poppy

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Embodiment Construction

[0032] Adopt the method of the present invention to carry out PCR detection from the poppy plant leaf of Longhui, Hunan, with not adding poppy template DNA as negative control, see figure 1 , the results showed that poppy leaves were positive, and a band of expected 635 bases was amplified. The amplified fragments were recovered and sequenced, and the sequencing results are shown in figure 2 , the sequence is shown in SEQ ID No.1. The amplified sequence was subjected to BLAST analysis, see image 3 , the results showed that the similarity between the amplified sequence and Papaver somniferum was 100%; the similarity between the amplified sequence and Papaver somniferum subsp was 99%. It shows that the method of the present invention can rapidly detect poppy plants.

[0033]

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Abstract

The invention provides a PCR detection method for poppy. The method comprises the following steps: carrying out PCR amplification on a to-be-detected sample by using eucaryon rDNA-ITS universal primer, cloning and sequencing PCR positive product, carrying out BLAST analysis on sequencing result, and finally identifying the detected sample. The detection method is different from other detection methods, the detection method can be used detecting and identifying poppy plant materials fast, so the detection method is beneficial for striking illegal poppy cultivation, restraining drug crime and guaranteeing peoples' live and security.

Description

technical field [0001] The invention relates to a detection method of poppy, in particular to a detection method of poppy plant material through PCR (polymerase chain reaction) method with rDNA-ITS universal primer. Background technique [0002] Poppy (Papaver somniferum) is a special economic crop for processing narcotic drugs and drugs (opium, morphine, heroin). Opium is processed from the juice cut from the capsule of poppy, also known as opium, opium, foreign medicine, local medicine, tobacco soil, opium, etc. In its raw state it is an opaque milky white sap that is found in various parts of the poppy plant, but is mostly concentrated in the active ingredient of the immature capsule. This milky white juice is oxidized as soon as it comes into contact with the air, turning into a dark brown viscous substance that is viscous to the touch and has a faint fragrance. Lumpy raw opium. A capsule can continuously secrete opium for several days, and can be cut up to 6 times, w...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q1/6844
Inventor 高必达黄欣张汝佳
Owner HUNAN AGRICULTURAL UNIV
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