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A dry stick device and a method for determining an analyte in a sample

An analyte, device technology, applied in the field of automatic or semi-automatic systems, which can solve the problems of expensive, increased risk of manufacturing failure, uneven impregnation, etc.

Inactive Publication Date: 2015-06-10
LATTEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

All these method steps are time consuming, expensive and increase the risk of manufacturing failure
Also the dust generated during the slitting and cutting steps can adversely affect the working environment
Yield losses may be encountered because porous materials have to be trimmed to avoid the use of material edges where uneven impregnation often occurs

Method used

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  • A dry stick device and a method for determining an analyte in a sample
  • A dry stick device and a method for determining an analyte in a sample
  • A dry stick device and a method for determining an analyte in a sample

Examples

Experimental program
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Effect test

preparation example Construction

[0133] Preparation of dry sticks

[0134] A comparison between the methods used to make traditional dry sticks and the methods used to make the dry stick structures of the present invention can be found at Figure 7 and 8 seen in.

[0135] Dry stick devices according to the invention may be prepared by any conventional method provided for the preparation of dry stick devices. In a preferred embodiment, the method for providing a dry stick device according to the invention comprises the following steps:

[0136] (i) providing a carrier comprising a cavity;

[0137] (ii) providing at least one dry reagent,

[0138] (iii) applying the at least one dry reagent to the cavity

[0139] And get this dry stick device.

[0140] Comparing the traditional method for the preparation of non-compartmented dry sticks in which one or more reagents are present in the same liner (i.e. the same porous material) with the above-mentioned method of the present invention, the following method...

example 1

[0294] Example 1: Preparation and testing of BHB dry sticks

[0295] Prepare the impregnation solution:

[0296] Dissolve the following reagents one at a time in 3400 mL of 0.1 M Phosphate Buffer pH 8.0:

[0297] 20.0g β-nicotinamide adenine dinucleotide (β-NAD+)

[0298] 9.0g nitro blue tetrazolium (NTB)

[0299] 32 mL of 5% Triton X-100 solution

[0300] 20.0g bovine serum albumin (BSA)

[0301] 400KU ascorbate oxidase (ASO-3 from Amano)

[0302] ·100KU diaphorase (Unitika)

[0303] 50KU hydroxybutyrate dehydrogenase (HBDH from Amano)

[0304] In order to produce a light yellow transparent solution.

[0305] To prepare dry sticks:

[0306] Fill the cavities of multiple separation rods with more than 10 μL of the freshly prepared solution in each cavity. The rods were then dried in a ventilated oven at 40° C. for 1 hour to produce a dry residue (about 250 μg) in each cavity, which stuck to the bottom of the cavity. Then, each separate dry stick obtained was placed...

example 2

[0314] Example 2: Preparation and testing of LDH dry sticks

[0315] Prepare impregnation solution A:

[0316] Dissolve the following reagents one at a time in 2600 mL of 0.1 M Phosphate Buffer pH 8.0:

[0317] 60.0g β-nicotinamide adenine dinucleotide (β-NAD + )

[0318] 30.0g lithium acetate

[0319] 3.0g PEG 20.000

[0320] 30.0g sucrose

[0321] 30 mL of 5% Triton X-100 solution

[0322] 30.0g bovine serum albumin (BSA)

[0323] 300KU Ascorbate Oxidase (ASO-3 from Amano)

[0324] ·600KU diaphorase (Unitika)

[0325] 3.0g nitro blue tetrazolium (NTB)

[0326] In order to produce a light yellow transparent solution.

[0327] Prepare impregnation solution B:

[0328] 450 g TRIS was dissolved in 1080 mL DI water to yield a clear colorless solution with a pH of about 12.

[0329] To prepare dry sticks:

[0330] exist First Fill the cavity of the stick with 8.4 μL of freshly prepared solution A above. The sticks were then dried in a ventilated oven at 40°C for ...

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PUM

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Abstract

A novel and improved dry stick construction for the determination of an analyte in a sample is provided. The dry stick device comprises: (i) a solid support comprising a cavity, and (ii) at least one dry reagent capable of reacting with the analyte, a derivative of said analyte or an indicator compound for said analyte to provide a detectable signal when in a moistened state, wherein the cavity comprises the at least one dry reagent. The present invention also provides an automated or semi-automated system. Additionally the present invention provides an automated or semi-automated system for optimising the production performance of a milk producing animal herd.

Description

field of invention [0001] The present invention relates to improved dry stick structures, methods of producing such dry sticks and their use in the analysis of analytes in samples. The invention also relates to an automatic or semi-automatic system comprising the improved dry stick structure. In addition, the present invention relates to an automatic or semi-automatic system for optimizing the performance of a dairy animal herd comprising the improved dry stick structure. Background of the invention [0002] Demand for rapid and reliable diagnostic tests is increasing. Most diagnostic tests in use today comprise a single layer of porous material, such as a filter pad, impregnated with one or more reagents capable of producing a detectable signal. Such diagnostic tests are non-partitioned in that the one or more reagents are present in a single layer of porous material. [0003] When using such non-compartmental dry sticks, the porous material is brought into contact with ...

Claims

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Application Information

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IPC IPC(8): G01N33/52G01N33/543G01N33/53A01J5/007
CPCA01J5/0131A01K1/12A01K11/006G01N33/521G01N33/525
Inventor 吉米·克劳森
Owner LATTEC
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