A device for separating tumor cells in blood fluid

Abstract

The invention provides a device for separating tumor cells in blood fluid, which comprises a fluid sample inlet, a first drive pump, a separation column and a fluid sample outlet that are sequentially connected in series and can circulate through a main circulation pipeline, and the first drive pump It is a peristaltic pump, and an anticoagulant physiological saline injection port and an anticoagulant antagonist injection port are also provided on the main circulation pipeline; more than two sub-filter columns are arranged in the separation column, and each sub-filter column All are provided with a track-etched membrane for separating tumor cells, the pore size of the track-etched membrane is 5-25um, and the track-etched membrane in each sub-filter column forms an independent three-dimensional filter channel; the device also includes a cell Collector; the device successively constitutes two operating paths of filtering and breaking away. The device is an omitted invention of an invention patent in the prior art. The device of the present invention can realize all its functions.

Description

technical field [0001] The invention relates to the field of cell separation in fluid, in particular to a device for separating tumor cells in blood fluid. Background technique [0002] In the prior art, when it is necessary to separate and detect cancer cells (circulating tumor cells, CTC) in the blood of living animals (including human beings), most of them extract a certain amount of blood, and then perform cell sorting and detection by methods such as density gradient centrifugation. count. Density gradient centrifugation is to use a certain medium to form a continuous or discontinuous density gradient in the centrifuge tube, place the cell suspension or homogenate on the top of the medium, and separate the cells by the action of gravity or centrifugal force field. But its shortcomings are obvious. Therefore, there is a need in the art for a new device and method for isolating and detecting the number of cancer cells in the blood of living animals. In recent years, a ...

AI Technical Summary

Problems solved by technology

However, the device corresponding to such a method can only separate cancer cells in a very small part of the blood of living animals at one time, but cannot effectively separate cancer cells in most or all of the blood of living animals, especially for larger living animals ( Such as the human body), this defect is particularly prominent

The reason is that in order to keep the animal in a normal living condition, it is impossible to radiate the whole body blood of a living animal to separate the cancer cells in it, so that the individual will die

In addition, because the number of tumor cells (CTCs) in the blood is extremely rare, if only a small amount of blood from live animals is extracted for separation and detection, the sensitivity of the separation and detection will be greatly reduced; and increasing the amount of processed blood will have a negative impact on the health and life of living animals. are threats

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