Standard apple extract as well as preparation and analysis method of standard apple extract
An apple extract, apple technology, applied in the direction of analyzing materials, material separation, measuring devices, etc., to achieve the effect of improving immunity, nourishing spleen and lung immunity
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[0061] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with the accompanying drawings and specific examples.
[0062] 1. Preparation of standard products for each compound
[0063] 1mg / mL standard solution: Accurately weigh 1.0mg of compound 1-10 standard substance (accurate to 0.001g) with an electronic balance, dissolve it in 0.2mL of methanol, and dilute to 1.0mL volumetric flask for cryopreservation.
[0064] Take 10μL, 40μL, 80μL, 150μL, 200μL of the above standard solutions respectively into 1.0mL volumetric flasks, dilute to volume with methanol, mix well and prepare the concentrations as 10mg / L, 40mg / L, 80mg / L, 150mg / L, 200mg / L series of standard solutions.
[0065] Inject 10 μL of samples respectively, and perform HPLC analysis under specific chromatographic conditions. Perform linear regression on the mass concentratio...
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