Multi-mode micro-imaging system and method based on LED array

Abstract

The invention discloses a multi-mode micro-imaging system and method based on an LED array. The LED array serves as a microscope system light source and generates controllable multi-angle illumination light and a controllable illumination pore diameter, and bright field imaging, dark field imaging and difference phase contrast imaging are achieved. The realizable multi-mode imaging includes the three imaging modes of bright field imaging, dark field imaging and difference phase contrast imaging, and while bright field imaging, dark field imaging and difference phase contrast imaging are achieved, it is unnecessary to add any additional optical elements into the imaging path of a traditional microscope; in this way, an optical system is greatly simplified, and the LED array enables the microscope to have the capacity of flexibly adjusting the illumination pore diameter, the illumination angle and light source coherence.

Description

technical field [0001] The invention belongs to optical imaging technology, in particular to an LED array-based multi-mode microscopic imaging system and a method thereof. Background technique [0002] Bright field imaging, dark field imaging and differential phase contrast imaging are three common microscopic imaging methods, and most microscopes currently have these three imaging modes. Bright-field imaging and dark-field imaging are the two most commonly used microscopic imaging methods. The difference between the two lies in the different illumination methods. If only the transmitted beam is allowed to pass through the objective diaphragm, it is called a bright field image; if only the diffracted beam is allowed to pass through the objective diaphragm, it is called a dark field image. Therefore, the bright field is to make the illumination aperture within the numerical aperture of the objective lens, and the field of view is bright; while the dark field is to allow the ...

AI Technical Summary

Problems solved by technology

[0009] Compared with the prior art, the present invention has significant advantages: (1) Solving the problems of complex optical path and difficult operation of traditional microscopes in bright field, dark field, and differential phase contrast imaging, adopting LED arrays as the illumination light source of the microscopic system , so as to achieve bright field, dark field and differential phase contrast imaging without adding any additional optical elements (such as annular diaphragm, phase plate, etc.) in the imaging light path of traditional microscopes, which greatly simplifies the optical system

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