Stable high-efficient method for ex-vivo preservation and growth recovery of strawberry germplasm resource
An in vitro preservation and growth recovery technology, applied in the agricultural field, can solve the problems of high manpower and material costs, prolong storage time, cross-contamination, etc., and achieve the effects of easy portability, prolonging the subculture cycle, and normal plant growth
Active Publication Date: 2015-07-22
JIANGSU ACAD OF AGRI SCI
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And when the amount of preservation is large, the cost of manpower and material resources consumed by multiple subcultures is also very high
Since the beginning of this century, researchers have successfully preserved germplasm resources such as Clover, Honeysuckle, Rhizoma Chuanxiong, Cassava, Atractylodes macrocephala, Dendrobium, Chrysanthemum and Lily in vitro. to report
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Embodiment 1
[0023] (1) the acquisition of sterile seedlings: select 80 parts of wild resources, 40 parts of cultivation resources (refer to the table below for specific materials, numbering cultivation resources 1 from the National Fruit Tree Germplasm Nanjing Strawberry Resource Garden, Jiangsu Academy of Agricultural Sciences, Horticultural Research Institute in November, 2011) -40, Wild Resources No. 41-120) Repeatedly save 8 copies of each resource, cut the shoot tips of newly grown stolons from the plants and rinse them with running water for 1.5-2 hours. On the sterile workbench, use 75 % medical alcohol for 30 s, then sterilized with 0.1% mercuric water for 8 min, and finally rinsed with sterile water for 3-4 times, peeled off the outer coating, inoculated the shoot tips on the medium ① or ②, placed at 25± The primary culture was carried out in a culture room at 2°C, the light intensity was about 2400 lx, and the daily light time was 14-16 h, and sterile seedlings were obtained in a...
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The invention belongs to the technical fields of plant tissue rapid propagation and germplasm resource preservation, and discloses a stable high-efficient method for ex-vivo preservation and growth recovery of a strawberry germplasm resource. The method specifically comprises steps of (1) acquirement of aseptic seedling, (2) growth culture, (3) growth inhibition; (4) low-temperature preservation, (5) inspection and growth recovery, (6) rooting and transplantation, and the like. The invention aims to solve the problem which exists in conventional ex-vivo preservation of the strawberry germplasm resource; a preservation condition and a preservation container are improved without adding any plant growth inhibitor; a subculture period can reach to 22-24 months and is prolonged by 4-20 months compared with the subculture period under a reported preservation period; and safety of preservation of the germplasm resource is effectively improved. The method is simple in operation, good in repeatability, and stable in technology, and is suitable for preservation of mass strawberry germplasm resources; exchange of germplasm resources at home and aboard becomes convenient; the problem that diseases, insect and pest propagation, and even dead seedling of the resources are taken place during a transportation process is avoided; and problems of large land occupation of preservation and high cost are solved.
Description
technical field [0001] The invention relates to a stable and efficient method for in vitro preservation and growth recovery of strawberry germplasm resources, belonging to the technical field of agriculture. Background technique [0002] Since the early 1980s, my country has established national fruit tree strawberry germplasm resource gardens, which are located in Beijing (in the Forestry and Fruit Tree Research Institute of Beijing Academy of Agriculture and Forestry) and Nanjing, Jiangsu (in the Horticultural Research Institute of Jiangsu Academy of Agricultural Sciences), marking the development of strawberry resources in my country. The introduction and preservation have entered a new stage. Some provincial scientific research units, colleges and universities, local research institutes or private units have also preserved some strawberry germplasm resources. At present, wild strawberries, local varieties, introduced varieties, and varieties (series) newly bred by domesti...
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IPC IPC(8): A01H4/00
Inventor 于红梅王静赵密珍袁华招钱亚明
Owner JIANGSU ACAD OF AGRI SCI