A stable and efficient method for in vitro preservation and growth recovery of strawberry germplasm resources
A technology of recovery growth and in vitro preservation, applied in the field of agriculture, can solve the problems of high manpower and material costs, prolong storage time, cross-contamination, etc., and achieve the effect of easy portability, prolonging the subculture cycle, and normal plant growth
Active Publication Date: 2017-05-24
JIANGSU ACAD OF AGRI SCI
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Problems solved by technology
And when the amount of preservation is large, the cost of manpower and material resources consumed by multiple subcultures is also very high
Since the beginning of this century, researchers have successfully preserved germplasm resources such as Clover, Honeysuckle, Rhizoma Chuanxiong, Cassava, Atractylodes macrocephala, Dendrobium, Chrysanthemum and Lily in vitro. to report
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Embodiment 1
[0023] (1) Acquisition of sterile seedlings: In November 2011, 80 copies of wild resources and 40 copies of cultivated resources were selected from the National Fruit Tree Germplasm Nanjing Strawberry Resource Garden in the Horticultural Research Institute of Jiangsu Academy of Agricultural Sciences (see the table below for specific materials, numbered cultivated resources 1 -40, wild resources No. 41-120) 8 copies of each resource were repeatedly preserved, and the newly grown stolon tip was cut from the plant and rinsed with running water for 1.5-2h. On the aseptic operation bench, first use 75 % medical alcohol for 30 seconds, then sterilized with 0.1% mercury liter water for 8 minutes, and finally rinsed with sterile water for 3-4 times, peeled off the outer bracts, inoculated the shoot tip on the medium ① or ②, and placed it at 25± The primary culture was carried out in a culture room with a light intensity of about 2400 lx and a daily light time of 14-16 hours at 2°C, and...
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The invention belongs to the technical fields of plant tissue rapid propagation and germplasm resource preservation, and discloses a stable high-efficient method for ex-vivo preservation and growth recovery of a strawberry germplasm resource. The method specifically comprises steps of (1) acquirement of aseptic seedling, (2) growth culture, (3) growth inhibition; (4) low-temperature preservation, (5) inspection and growth recovery, (6) rooting and transplantation, and the like. The invention aims to solve the problem which exists in conventional ex-vivo preservation of the strawberry germplasm resource; a preservation condition and a preservation container are improved without adding any plant growth inhibitor; a subculture period can reach to 22-24 months and is prolonged by 4-20 months compared with the subculture period under a reported preservation period; and safety of preservation of the germplasm resource is effectively improved. The method is simple in operation, good in repeatability, and stable in technology, and is suitable for preservation of mass strawberry germplasm resources; exchange of germplasm resources at home and aboard becomes convenient; the problem that diseases, insect and pest propagation, and even dead seedling of the resources are taken place during a transportation process is avoided; and problems of large land occupation of preservation and high cost are solved.
Description
technical field [0001] The invention relates to a stable and efficient method for in vitro preservation and growth recovery of strawberry germplasm resources, which belongs to the field of agricultural technology. Background technique [0002] Since the early 1980s, my country has established national fruit tree strawberry germplasm resource gardens, which are respectively located in Beijing (in the Institute of Forestry and Fruit Trees, Beijing Academy of Agriculture and Forestry Sciences) and Nanjing, Jiangsu (in the Institute of Horticulture, Jiangsu Academy of Agricultural Sciences), marking the development of strawberry resources in my country. Introduction, preservation and other aspects have entered a new stage. Some provincial scientific research units, colleges and universities, local research institutes or private units have also preserved some strawberry germplasm resources. At present, wild strawberry, local varieties, introduced varieties, and varieties (lines) n...
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IPC IPC(8): A01H4/00
Inventor 于红梅王静赵密珍袁华招钱亚明
Owner JIANGSU ACAD OF AGRI SCI