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A kind of acid-soluble chitosan microsphere and preparation method thereof

A technology of chitosan microspheres and water-soluble chitosan, which is applied in the field of monodisperse acid-soluble chitosan microspheres and its preparation, can solve the problems of difficult monodispersity and achieve good monodispersity and good Sphericity and Structural Integrity, Effect of Good Sphericity

Active Publication Date: 2017-07-14
四川迈可隆生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because chitosan has the characteristics of unique biological activity, non-toxicity, good biocompatibility and easy degradation, it is usually used as a drug carrier to prepare chitosan microspheres or microcapsules, but there is no preparation of chitosan at present. It is reported that microspheres are used to replace red blood cells as a standard for the calibration of blood cell analysis instruments, mainly because it is difficult to prepare chitosan into microspheres with a particle size comparable to that of red blood cells and good monodispersity by existing methods

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  • A kind of acid-soluble chitosan microsphere and preparation method thereof
  • A kind of acid-soluble chitosan microsphere and preparation method thereof
  • A kind of acid-soluble chitosan microsphere and preparation method thereof

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Embodiment 1

[0035] In the present embodiment, the preparation method of chitosan microspheres is as follows:

[0036] (1) Preparation of spray liquid and receiver liquid

[0037] Preparation of spray liquid: Dissolve water-soluble chitosan in deionized water to form a chitosan solution, then adjust the pH value of the chitosan solution to between 6.3 and 6.4 with a sodium hydroxide aqueous solution with a concentration of 1mol / L, and The chitosan solution after adjusting the pH value is centrifuged and filtered with filter paper to form a spray liquid; the mass ratio of the water-soluble chitosan to deionized water is 0.02:1;

[0038] Preparation of the receiving solution: mix glycerol polyricinoleate, tetramethylethylenediamine and the solvent evenly, then add terephthalaldehyde, stir until the terephthalaldehyde is completely dissolved to form the receiving solution; the solvent is toluene and The mixed solution of n-hexanol, the volume ratio of toluene and n-hexanol is 1:1, the amount...

Embodiment 2

[0044] In the present embodiment, the preparation method of chitosan microspheres is as follows:

[0045] (1) Preparation of spray liquid and receiver liquid

[0046] Preparation of spray liquid: Dissolve water-soluble chitosan in deionized water to form a chitosan solution, then adjust the pH value of the chitosan solution to between 6.4 and 6.5 with a sodium hydroxide aqueous solution with a concentration of 2 mol / L. The chitosan solution after adjusting the pH value is centrifuged and filtered with filter paper to form a spray liquid; the mass ratio of the water-soluble chitosan to deionized water is 0.03:1;

[0047] Preparation of the receiving solution: mix glycerol polyricinoleate, tetramethylethylenediamine and the solvent evenly, then add terephthalaldehyde, stir until the terephthalaldehyde is completely dissolved to form the receiving solution; the solvent is toluene and The mixed solution of n-hexanol, the volume ratio of toluene and n-hexanol is 1:1, the amount of...

Embodiment 3

[0053] In the present embodiment, the preparation method of chitosan microspheres is as follows:

[0054] (1) Preparation of spray liquid and receiver liquid

[0055] Preparation of spray liquid: Dissolve water-soluble chitosan in distilled water to form a chitosan solution, and then adjust the pH value of the chitosan solution to 6.4 to 6.5 with a sodium hydroxide aqueous solution with a concentration of 1mol / L. The chitosan solution after the pH value is centrifuged and filtered with filter paper to form a spray liquid; the mass ratio of the water-soluble chitosan to distilled water is 0.02:1;

[0056] Preparation of the receiving solution: mix glycerol polyricinoleate, tetramethylethylenediamine and solvent evenly, then add terephthalaldehyde, stir until the complete dissolution of terephthalaldehyde forms the receiving solution; the solvent is toluene and The mixed solution of n-hexanol, the volume ratio of toluene and n-hexanol is 1:1, the amount of polyricinoleic acid g...

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Abstract

The invention provides an acid-soluble chitosan microsphere, which is formed by the cross-linking reaction of chitosan and terephthalaldehyde, the particle diameter of the microsphere is 6-11 μm, and the variation coefficient of the microsphere is 2.7 %~5%. Its preparation method is as follows: (1) Prepare spray liquid and receiving liquid; (2) In a constant temperature environment of 25-45°C, add the spray liquid at the same temperature as the constant temperature environment into the syringe of the electrospinning equipment, and then pump Push in the metal needle, apply high-voltage static electricity at the metal needle, and the spray liquid will form a spray droplet. The container containing the receiving liquid is used to receive the spray droplet under stirring conditions. The chitosan in the spray droplet and the receiving Terephthalaldehyde in the solution is completely cross-linked to obtain chitosan microspheres; (3) washing the obtained chitosan microspheres with isopropanol to remove the receiving liquid on the surface of the microspheres, and then washing with water to remove isopropanol.

Description

technical field [0001] The invention belongs to the field of chitosan microspheres, in particular to monodisperse acid-soluble chitosan microspheres with the size of red blood cells and a preparation method thereof. Background technique [0002] With the widespread application of electronic technology, flow cytometry, laser technology and electronic computers in medical testing instruments, the degree of automation of blood cell analyzers (flow cytometers) has been continuously improved and widely used in clinical practice. Since the data measured by the blood cell analyzer are relative values, the instrument system must be calibrated or calibrated before use. At present, the standard product used to calibrate the blood cell analyzer is human or chicken red blood cells (see U.S. Pat. No. 4489162), but extracting red blood cells from mammalian blood has complicated preparation process, high biological risk and There are problems such as high cost, and the shelf life of such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14G01N1/28B01J13/02A61K47/36
Inventor 巨晓洁王小雪褚良银谢锐汪伟刘壮
Owner 四川迈可隆生物科技有限公司