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Method for infecting mesenchymal stem cells by Runx2 recombinant lentiviruses to express osteoblastic genes

A bone marrow mesenchymal and recombinant lentivirus technology, which is applied in genetic engineering, plant gene improvement, botany equipment and methods, etc., to achieve the effect of increased expression level, good cell growth, and improved expression level

Inactive Publication Date: 2015-07-29
THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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  • Abstract
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  • Application Information

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Problems solved by technology

Currently, there is no method to use Runx2 recombinant lentivirus to infect bone marrow mesenchymal stem cells to express osteogenic genes

Method used

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  • Method for infecting mesenchymal stem cells by Runx2 recombinant lentiviruses to express osteoblastic genes
  • Method for infecting mesenchymal stem cells by Runx2 recombinant lentiviruses to express osteoblastic genes
  • Method for infecting mesenchymal stem cells by Runx2 recombinant lentiviruses to express osteoblastic genes

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Embodiment Construction

[0021] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0022] The lentiviral vector is obtained by transformation of human immunodeficiency virus (humanimmunodeficiency virus, HIV). The lentiviral vector system includes expression vector and packaging plasmid. Integrate the required genetic information. Compared with retroviruses, lentiviral vectors have obvious advantages: lentiviruses can infect non-dividing cells, allowing cells to maintain their characteristics; lentiviruses will not cause changes in gene expression of target cells, making them avoid secondary cancers. Compared with adenoviral vectors, lentiviral ...

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Abstract

The invention applies to the technical field of biological medicine and provides a method for infecting infecting mesenchymal stem cells by Runx2 recombinant lentiviruses to express osteoblastic genes. The method comprises steps as follows: Runx2 gene extraction and plasmid construction are performed; the Runx2 recombinant lentiviruses are packaged; the Runx2 recombinant lentiviruses infect the mesenchymal stem cells, so that infected mesenchymal stem cells capable of expressing the osteoblastic genes are obtained.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for expressing osteogenic gene by infecting bone marrow mesenchymal stem cells with Runx2 recombinant lentivirus. Background technique [0002] Large bone defects have always been a difficult problem in the treatment of trauma orthopedics. At present, traditional autologous bone, allogeneic bone, and artificial bone materials are difficult to effectively treat large-area bone defects. Bone tissue engineering plays an important role in promoting bone healing by integrating scaffold materials, seed cells, and active factors to improve the osteogenic activity of artificial bone materials. [0003] Bone marrow mesenchymal stem cells are a type of non-hematopoietic stem cells that exist in the bone marrow and have multi-directional differentiation potential. Under certain induction conditions, they can differentiate into osteoblasts, chondrocytes, adipocytes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/867C12Q1/70
Inventor 王大平熊建义张巨峰付志杰李庆庆廖福朋
Owner THE SECOND PEOPLES HOSPITAL OF SHENZHEN
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