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Bacillus subtilis strain as well as metabolite and application of bacillus subtilis strain

A technology of Bacillus subtilis and metabolites, applied in application, bacteria, microorganisms and other directions, can solve the problems of easy inactivation of protease and high cost of protease, and achieve the effects of abundant sources, simple fermentation conditions, and no toxic and side effects.

Inactive Publication Date: 2015-08-12
HUNAN AGRI BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current domestic and foreign related research, although the use of protease to hydrolyze protein materials can improve its inhibitory activity against weeds, the enzymatic hydrolysis reaction of protease has relatively strict requirements on temperature, pH and other conditions, and protease is prone to inactivation under adverse conditions. In addition, the use of protease also has the problem of high cost

Method used

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  • Bacillus subtilis strain as well as metabolite and application of bacillus subtilis strain
  • Bacillus subtilis strain as well as metabolite and application of bacillus subtilis strain

Examples

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Embodiment 1

[0029] The bacillus subtilis of the present embodiment is bacillus subtilis L-5 (Bacillus subtilis L-5), the strain was collected on February 2, 2015 at the General Microbiology Center of China Committee for Culture Collection of Microorganisms (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences ) preservation, the strain preservation number is CGMCC No: 10505.

[0030] The Bacillus subtilis L-5 described in the present embodiment is separated, screened, identified by the following methods:

[0031] (1) Isolation and screening of strains: Weigh 5.0g of soy sauce residue (collected from a local soy sauce factory in Changsha, Hunan), add it to 100ml of sterile normal saline, shake it for 2 hours, and then dilute it with normal saline; the dilution factor is 10 5 ~10 8 100 microliters of each bacterial solution were evenly spread on the LB solid medium, cultured upside down at 37°C for ...

Embodiment 2

[0037] The metabolite of Bacillus subtilis of the present invention, i.e. the preparation of bacterial strain Bacillus subtilis L-5 soybean protein fermentation liquid, specifically comprises the following implementation steps:

[0038] (1) Cultivate the strain Bacillus subtilis L-5 on LB solid medium for 24 hours, and inoculate it in 100ml / 250ml seed medium. The formula of the seed medium is 10g of tryptone, 5g of yeast extract, NaCl 10 grams, 1000 ml of distilled water, pH value 7.0, sterilized at 121°C for 20 minutes, cultured at 30°C for 12-18 hours to logarithmic phase after inoculation;

[0039] (2) Inoculate the seed liquid of the logarithmic strain strain in the fermentation medium, the inoculum amount is 4wt%, and the fermentation medium formula is: 20 grams of soybean protein isolate powder, 10 grams of NaCl, 1000 ml of distilled water, pH value 7.0, extinguished at 121 °C Bacteria for 20 minutes, cooled and then inoculated, then cultured with shaking at 30°C and 150...

Embodiment 3

[0041] In this example, only in step (2), inoculate the seed solution of the logarithmic strain in the fermentation medium, and culture it with shaking at 30°C and 150 rpm for 0, 24, 48, 96, and 120 hours, respectively. Other operation steps are identical with embodiment 2. The fermented liquid obtained at different culture times was used in Example 4 to study its inhibitory effect on barnyardgrass rooting activity.

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Abstract

The invention provides a bacillus subtilis strain as well as a metabolite and the application of the bacillus subtilis strain. The bacillus subtilis L-5 is collected in China General Microbiological Culture Collection Center on February 02, 2015, with the collection number CGMCC No: 10505. The invention also provides the metabolite of the bacillus subtilis and the application of the bacillus subtilis for preventing and removing paddy field weeds. The bacillus subtilis L-5 is used for fermenting soybean isolate protein, and the fermented liquid has obvious inhibition activity for barnyard grass and in particular for the rooting of the newly germinated barnyard grass; the fermented liquid has no influence on the root system of mature crops. If the fermented liquid is applied to weeding, the fermented liquid has the remarkable characteristics of environmental friendliness, no drug resistance of weeds, safety to crops and the like and has favorable application prospect.

Description

technical field [0001] The present invention relates to a strain of Bacillus subtilis and its metabolite and its application, in particular to a strain of Bacillus subtilis with herbicidal activity and its metabolite soybean protein fermentation liquid and its application, which belongs to natural plant materials and is used for agricultural plant protection and control of crops Technical field of weeds. Background technique [0002] At present, the biological control of harmful organisms in the planting industry is mainly aimed at plant diseases and pest damage, while the biological control methods for weeds are seldom used. [0003] Weed damage is one of the main problems affecting agricultural production. Over the years, chemical control has been the main solution. However, with the long-term and excessive use of chemical herbicides, the environmental problems and impact on crop quality have become increasingly serious. It is an effective way to solve this problem by see...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/06A01N63/02A01P13/02C12R1/125
Inventor 周小毛罗坤柏连阳李振兴邬腊梅彭迪刘佳
Owner HUNAN AGRI BIOTECH RES CENT
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