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A kind of preparation method of the graphene oxide composite material modified by carboxymethyl chitosan

A technology of carboxymethyl chitosan and composite materials, which is applied in the field of preparation of graphene oxide composite materials, can solve the problems of chitosan application limitations, and achieve the effect of mild experimental conditions and simple operation

Inactive Publication Date: 2017-04-12
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chitosan is insoluble in water and can only be dissolved in some acidic aqueous solutions, which greatly limits the application of chitosan

Method used

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  • A kind of preparation method of the graphene oxide composite material modified by carboxymethyl chitosan
  • A kind of preparation method of the graphene oxide composite material modified by carboxymethyl chitosan
  • A kind of preparation method of the graphene oxide composite material modified by carboxymethyl chitosan

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Graphene oxide GO (50 mg) was dissolved in 10 mL of water, activators EDC (30 mg) and NHS (18 mg) were added, and the reaction was stirred at room temperature for 2 h. During the reaction, 5 mL of an aqueous solution containing CMC (75 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each volume is 2 L, and then dialyzing with distilled water for 7 times, each volume is 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0027] (2) Dissolve 40 mg of GO-CMC from the above product in an appropriate amount of distilled water, add 1.2 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir for 3 h at room temperature.

[0028] (3) Add 360uL of triethylamine to the above product, mix and stir for 30min, add 240uL of acetic anhydride and mix, and rea...

Embodiment 2

[0031] (1) Graphene oxide GO (100 mg) was dissolved in 25 mL of water, and activators EDC (45 mg) and NHS (35 mg) were added, and the reaction was stirred at room temperature for 2 h. During the reaction, 20 mL of an aqueous solution containing CMC (200 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each volume is 2 L, and then dialyzing with distilled water for 7 times, each volume is 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0032] (2) Dissolve 30 mg of GO-CMC from the above product in an appropriate amount of distilled water, add 1 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir for 3 h at room temperature.

[0033] (3) Add 280uL triethylamine to the above product, mix and stir for 20min, add 160uL acetic anhydride and mix, and reac...

Embodiment 3

[0036] (1) Graphene oxide GO (78 mg) was dissolved in 10 mL of water, activators EDC (45 mg) and NHS (40 mg) were added, and the reaction was stirred at room temperature for 2.5 h. During the reaction, 20 mL of an aqueous solution containing CMC (120 mg) was added dropwise, and the reaction was stirred at room temperature for 24 h. After the reaction, the solution was dialyzed with a dialysis bag (MW=100,000). After dialyzing twice with PBS, each volume is 2 L, and then dialyzing with distilled water for 7 times, each volume is 2 L, to remove impurities and by-products in the solution. The obtained product was freeze-dried to synthesize GO-CMC.

[0037] (2) Dissolve 20 mg of GO-CMC from the above product in an appropriate amount of distilled water, add 0.8 mg of fluorescein isothiocyanate FITC in DMSO solution, and stir for 3 h at room temperature.

[0038] (3) Add 200uL triethylamine to the above product, mix and stir for 30min, add 115uL acetic anhydride and mix, and react...

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Abstract

The invention relates to a preparation method of a carboxymethyl chitosan modified graphene oxide composite material; the preparation method includes the steps: (1) under the action of activating agents EDC and NHS, allowing graphene oxide dispersed in water to generate chemical bond connection with CMC, carrying out a reaction for 24 h at room temperature, to obtain a suspension with relatively good micro-particle dispersity, dialyzing, freeze-drying, and thus synthesizing GO-CMC; and (2) dissolving the obtained product in distilled water, adding an appropriate amount of fluorescein FITC; and acetylating the product, carrying out a stirring reaction for 24 h at room temperature, dialyzing, freeze-drying, and thus synthesizing the graphene oxide GO-CMC-FI-Ac composite material. The preparation method is simple to operate, and the product is easy to handle; and the composite material can be applicable to drug sustained release and cell imaging, and the cost is low.

Description

technical field [0001] The invention belongs to the field of preparation of graphene oxide composite materials, in particular to a preparation method of a graphene oxide composite material modified by carboxymethyl chitosan. Background technique [0002] In 2004, Geim and Novoselov of the University of Manchester, UK, used the tape stripping technique to successfully prepare for the first time a new two-dimensional atomic crystal-graphene composed of a single atomic layer of carbon atoms connected by sp2 hybridization. The discovery overturns scientists' predictions that ideal 2D crystalline materials cannot exist at room temperature due to thermodynamic instability. And due to the excellent mechanical, electrical, and optical properties of graphene, graphene-based composite materials have become a hot spot in the field of graphene applications, which have broad applications in energy storage, liquid crystal devices, electronic devices, biological materials and other fields....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/23
Inventor 朱利民潘琪霞陶磊杨卉卉吕瑶
Owner DONGHUA UNIV
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