Special slide for comet experiment

Abstract

The invention discloses a special slide for comet experiment. The special slide comprises a base plate and edge plates, wherein the base plate is a strip flat plate; more than two circular groove holes are formed in the upper surface of the base plate, and the bottom part of each circular groove hole is concave and flat; the vertical edge plates are arranged at the two ends of the base plate; the base plate and the edge plates are of an integral structure which is made of transparent polystyrene plastic. The special slide has the advantages that the structure is simple, and the convenience in use is realized; the material of glass is replaced by the transparent polystyrene, the easiness in reaction with alkaline liquid is avoided, the repeated use is realized, and the resource is saved.

Description

technical field [0001] The invention relates to a slide specially used for comet experiments. Background technique [0002] The comet assay is a common method for sensitive detection of DNA damage and repair at the single-cell level. [0003] The currently commonly used slides for comet experiments have the following deficiencies: [0004] 1. At present, the commonly used slides are made of glass, which is easy to react with lye during the experiment and will have a certain impact on the experimental results; [0005] 2. The glass slides currently on the market are only 2-well, 3-well and 96-well. The slots are made by directly digging holes in the middle of the hydrophobic barrier and attaching them to the slide. Before the gel is laid until it is not solidified, the liquid gel is easy to overflow from the gap between the upper water barrier and the lower glass slide to form a circular hole, so that the thickness of the final gel is not enough and uneven, and it can be us...

AI Technical Summary

Problems solved by technology

[0004] 1. At present, the commonly used slides are made of glass, which is easy to react with lye during the experiment and will have a certain impact on the experimental results;

[0005] 2. The glass slides currently on the market are only 2-well, 3-well and 96-well. The slot holes are made by digging holes directly in the middle of the hydrophobic barrier and attaching them to the slide.

Before the gel is laid until it is not solidified, the liquid gel is easy to overflow from the gap between the upper water barrier and the lower glass slide to form a circular hole, so that the thickness of the final gel is not enough and uneven, and it can be used at the same time. Cause the glue surface to be uneven;

[0006] 3. Ordinary glass slides are sheet-shaped, which is inconvenient to take and place;

[0007] 4. At present, the slides for comet experiments on the market are relatively expensive, and they are disposable. They are not universal, waste resources, cause environmental pollution, and are not suitable for the development of the current conservation-oriented society.

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