Detection method and application of genotype at rs492594

A genotype and genome technology, applied in the field of biomedicine, can solve the problems of patients with adverse reactions, curative effect, increased fracture risk, weight gain, etc.

Active Publication Date: 2015-09-09
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also adverse effects leading to edema, weight gain, increased fracture risk, and heart failure
The indications for pioglitazone are all type 2 diabetes, but due to the heterogeneity of the etiology and pathogenesis of type 2 diabetes, if it is not subdivided, there will inevitably be a small number of patients with adverse reactions or poor curative effect, resulting in increased medical expenses for patients. Physical and mental harm caused by adverse reactions

Method used

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  • Detection method and application of genotype at rs492594
  • Detection method and application of genotype at rs492594
  • Detection method and application of genotype at rs492594

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1, the study of pioglitazone on the curative effect of different genotype diabetes patients

[0064] Ethics statement

[0065] This study was approved by the Ethics Committee (IRB) of Peking University Health Science Center, and all patients with prediabetes signed an informed consent.

[0066] 1. Collection of clinical data and biological samples

[0067] Carry out clinical trials of pioglitazone drugs, establish research cohorts, and collect clinical data and biological samples at various stages.

[0068] The study is a randomized, drug-blind, parallel, placebo-controlled prospective multicenter clinical trial. According to the WHO1999 diagnostic criteria, 1903 pre-diabetic patients were included, of which 953 were treated with pioglitazone (the dose of pioglitazone was 30mg / d, and all subjects completed one-year follow-up. The baseline and follow-up data of the subjects were collected, including the height of the patients. , body weight, waist circumfer...

Embodiment 2

[0095] Example 2, Genotype detection of rs492594 site

[0096] 1. Primer design for genotype detection of rs492594 locus

[0097] 1. Detection principle

[0098] The rs492594 site is usually typed by chip detection and flight mass spectrometry. This method is complex and suitable for multi-site and high-throughput research, but for studies with fewer typing sites and fewer cases, the experimental The conditions and costs are relatively high, which is not conducive to the development of clinical testing. Based on the results of this study, it is necessary to design a convenient and practical kit.

[0099] Restriction fragment length polymorphism (RFLP), as the first generation of molecular biology markers, is widely used in biological research, with low cost and simple operation, and can be operated by general laboratories. The experimental principle is: DNA restriction endonuclease has the activity of recognizing a specific DNA sequence and cutting the DNA double strand at ...

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Abstract

The invention discloses a detection method and application of a genotype at rs492594. The invention provides an application of a product for detecting a genotype of an rs492594SNP (Single Nucleotide Polymorphism) locus in a human genome or a product for detecting a genotype of an rs570876SNP locus in the human genome to the preparation of a product with the effect of reducing the blood glucose of people after the prediction of pioglitazone intervention. The invention proves that the genotype at the rs492594 of a diabetic is related to the blood glucose reducing effect of pioglitazone, the detection on the rs492594 can be used for screening type-2 diabetics who are relatively sensitive to the pioglitazone, and a proper blood glucose reducing drug can be selected according to the genotype at the rs492594 of the diabetic, so that the individualized treatment level is improved. The invention further discloses an RFLP (Restriction Fragment Length Polymorphism) method of the genotype at the rs492594. Through the method, the genotype of the rs492594SNP locus can be reliably, simply, conveniently, economically and quickly identified.

Description

technical field [0001] The invention relates to a genotype detection method of rs492594 and its application, belonging to the field of biomedicine, Background technique [0002] Thiazolidinedione drugs, also known as insulin sensitizers, are commonly used oral hypoglycemic drugs in clinical practice. Thiazolidinedione drugs include pioglitazone, troglitazone, rosiglitazone, etc. These drugs have a similar chemical structure, that is, 2,4-thiazolidinedione structure, and the difference between different drugs is that they have different side chain substitutions base. However, the safety of these compound drugs is not the same, and only pioglitazone is still widely used clinically. Thiazolidinediones are peroxisome proliferator-activated receptor gamma (PPARγ) agonists. Structural changes occur after PPARγ is activated, and it can recruit a variety of co-activators to bind with it and play different biological roles, including increasing insulin sensitivity in peripheral ti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6827C12Q2600/106C12Q2600/156
Inventor纪立农刘昭前
OwnerPEOPLES HOSPITAL PEKING UNIV