Jettisonable one-step electro-deposition immune-biosensor for detecting histamine and preparation method of immune-biosensor

A biosensor and electrodeposition technology, applied in the field of biosensors, can solve the problems of small molecular weight of histamine, only one antigen active site, and it is difficult to directly detect histamine, and achieve good viscosity, good electrocatalytic activity, and obvious stability sexual effect
CN104897748AActive Publication Date: 2015-09-09深圳深镧科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
深圳深镧科技有限公司
Publication Date
2015-09-09

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Abstract

The invention belongs to the technical field of biosensors, and specifically discloses a jettisonable one-step electro-deposition immune-biosensor for detecting histamine and a preparation method of the immune-biosensor. The immune-biosensor adopts an electrode coated with a prussian blue, chitosan and nanogold composite film, wherein histamine coating antigen is fixed on the electrode, the molar concentration ratio of nanogold to prussian blue in the composite film is 1:2000 to 1:20, and the massic volume content of chitosan is 0.01-0.05%. Furthermore, the composite film is formed on the surface of the electrode through a one-step electrode deposition manner, the deposition step is simpler than a preparation step of the conventional electrode modified by high-molecular polymer, and the antigen binding site can be easily exposed out. According to the electro-deposition immune-biosensor, competition immunoreaction and a biosensor are combined, and a series of property characteristics such as a histamine detection working curve can be obtained according to needs, and the jettisonable one-step electro-deposition immune-biosensor has the advantages of simplicity in operation, high sensitivity, rapidness, convenience and the like, and can be used for on-site rapid detection for histamine in a sample.
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Description

technical field

[0001] The invention belongs to the technical field of biosensors, and more specifically relates to a throwable one-step electrodeposition immunobiological sensor for detecting histamine and a preparation method thereof. Background technique

[0002] my country is rich in fish resources and is also a big country in the production and consumption of aquatic products. Aquatic products and their processing play an important role in the national economy and consumption. Histamine is formed by the decarboxylation reaction of free histidine under the catalysis of histidine decarboxylase. It is a toxic biogenic amine and widely exists in various organisms and foods, mainly aquatic products. Especially pelagic fish with green skin and red meat and their processed products. Studies have shown that excessive intake of histamine can cause a series of allergic reactions such as headache, nausea, nausea and vomiting, diarrhea, heart palpitations, itchy rash, and changes ...

Claims

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