A method for preparing pineapple flavor for tobacco by treating pineapple juice with Arthrobacter
A technology of pineapple juice and Arthrobacter, which is applied in the field of tobacco flavors, can solve the problems of easy occurrence of muddy sediment, residual negative effects, slow filtration speed, etc., and achieve the effects of enriching cigarette aroma, improving moisture retention, and convenient filtration
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Embodiment 1
[0018] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juice extractor to beat the pineapple, collect the slurry, and sterilize it for 15 minutes to obtain pineapple juice;
[0019] 2. Preparation of Arthrobacter preparation: According to the Arthrobacter preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water mass ratio of 1:100, the Arthrobacter biological preparation solution was prepared;
[0020] 3. Inoculation: under aseptic conditions, the Arthrobacter preparation is inoculated in the sterilized pineapple juice, and the inoculation amount is 0.005% (weight ratio);
[0021] 4. Fermentation: ferment the inoculated pineapple juice for 12 days at a pH value of 5, a stirring speed of 50 rpm, an aeration rate of 0.2 VVM, and a fermentation temperature of 20°C;
[0022] 5. Separation and purification: Centrifuge the fermented liquid obtained after fermentation a...
Embodiment 2
[0028] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juicer to beat the pineapple, collecting the slurry, and sterilizing it for 20 minutes to obtain pineapple juice;
[0029] 2. Preparation of Arthrobacter preparation: According to the Arthrobacter preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water mass ratio of 1:100, the Arthrobacter biological preparation solution was prepared;
[0030] 3. Inoculation: under aseptic conditions, the Arthrobacter preparation is inoculated in the sterilized pineapple juice, and the inoculation amount is 0.01% (weight ratio);
[0031] 4. Fermentation: ferment the inoculated pineapple juice for 8 days at a pH value of 6, a stirring speed of 80 rpm, an aeration rate of 0.5 VVM, and a fermentation temperature of 35°C;
[0032] 5. Separation and purification: centrifuge the fermented liquid obtained after fermentation at a ce...
Embodiment 3
[0038] 1. Preparation of pineapple juice: Take 500g of fresh pineapple, add 5 times the volume of water and wash it twice. Using a juicer to beat the pineapple, collecting the slurry, and sterilizing it for 30 minutes to obtain pineapple juice;
[0039] 2. Preparation of Arthrobacter preparation: According to the Arthrobacter preparation (purchased from Yada Group Science, Industry and Trade Co., Ltd.) and sterile water mass ratio of 1:100, the Arthrobacter biological preparation solution was prepared;
[0040] 3. Inoculation: under aseptic conditions, the Arthrobacter preparation is inoculated in the sterilized pineapple juice, and the inoculation amount is 0.05% (weight ratio);
[0041] 4. Fermentation: ferment the inoculated pineapple juice for 5 days at a pH value of 8, a stirring speed of 100 rpm, an aeration volume of 1 VVM, and a fermentation temperature of 45°C;
[0042] 5. Separation and purification: centrifuge the fermented liquid obtained after fermentation at a c...
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