The invention provides a microcapsule double-aqueous-phase extraction method of tobacco clove oil, and applications thereof. The extraction method comprises the following steps: putting cloves into an ultrasonic extraction device, performing ultrasonic extraction by adopting an ethanol water solution as a solvent to obtain a clove extracting liquid, filtering, and adding a cyclodextrin capsule wall material and inorganic salt or inorganic base, evenly stirring and standing and layering; filtering and separating a cyclodextrin microcapsule layer, adding absolute ethanol into the cyclodextrin microcapsule layer and stirring for 30-60 min, and releasing a capsule core; and then filtering and separating the mixed solution to obtain an ethanol layer, and performing decompression distillation until no ethanol is distilled, thus obtaining the tobacco clove oil. The purification method is simple, mild in conditions, and environment-friendly, the purified clove oil selectively retains the characteristic component eugenol of cloves, another two main components, eugenol acetate and caryophyllene, obtained through common vapor distillation can be removed, the invention provides a method for separating and purifying eugenol from clove oil, and the eugenol is used for covering up miscellaneous gas in a cigarette and improving the aroma quality.