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Cox‑1 gene polymorphism associated with aspirin resistance and its application

A technology of aspirin, -ACAGTCTGTGATGTGAG-3, applied in the field of medical disease treatment drug selection, can solve the problem of uncertain gene polymorphism and achieve the effect of reducing the recurrence rate of stroke

Inactive Publication Date: 2017-10-17
武汉欧瑞康安生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to incomplete statistics, clinical aspirin resistance is as high as 40% of patients, but because there are many targets that affect the action of aspirin, so far, the gene polymorphisms related to aspirin resistance are still uncertain

Method used

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  • Cox‑1 gene polymorphism associated with aspirin resistance and its application
  • Cox‑1 gene polymorphism associated with aspirin resistance and its application
  • Cox‑1 gene polymorphism associated with aspirin resistance and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Discovery of the COX-1 gene polymorphism (A842G) associated with aspirin resistance:

[0028] The data of stroke patients from 2010 to 2014 were retrieved from the stroke database, and according to the diagnostic criteria of ischemic stroke, patients diagnosed with ischemic stroke were screened, and aspirin was further selected for secondary prevention (secondary prevention is for patients with cerebral palsy). For patients with stroke symptoms or after stroke, these people need to prevent reoccurrence of stroke) as the total sample (5033 people in total).

[0029] 1. Determine the number of people with stroke recurrence in the total sample, and explore the relationship between the COX-1 (A842G) gene polymorphism and stroke recurrence in the total sample (Table 2);

[0030] 2. Among the total samples, patients who came to the hospital for reexamination (including relapsed and non-relapsed patients, a total of 3429 patients), collected peripheral blood from the patients,...

Embodiment 2

[0046] Application of the primers for the COX-1 gene polymorphism site (A842G) related to aspirin resistance in the preparation of a kit for screening aspirin resistance patients:

[0047] All patients with ischemic stroke for the first time were given aspirin for secondary prevention, and the patients were followed up 3 months later to detect the ARU and stroke recurrence of each patient. At the same time, the peripheral blood of the patients was collected to detect the presence of COX-1 (A842G) polymorphism by gene sequencing, and the incidence of aspirin resistance in mutant and wild-type patients was analyzed.

[0048] The study began in August 2013, and the deadline for case inclusion was August 2014. According to the diagnostic criteria for ischemic stroke, patients diagnosed with ischemic stroke were selected as the research objects, and the following tasks were completed:

[0049] 1. According to the guidelines for the secondary prevention of ischemic stroke, aspirin w...

Embodiment 3

[0059] Discovery of other genetic polymorphisms associated with aspirin resistance:

[0060] 1) Discovery of the GPⅠa gene polymorphism (C807T) associated with aspirin resistance:

[0061] With the same stroke database, blood sample and method as in Example 1, detect the relationship between the polymorphism of the GPⅠa (C807T) gene and stroke recurrence in the total sample (5033 people in total, the same as in Example 1) (Table 6); The relationship between the polymorphism of the GPIa (C807T) gene and ARU ≥ 550 in patients (including relapsed and non-relapsed patients, a total of 3429 people, the same as in Example 1) was reexamined (Table 5).

[0062] The results showed that among patients with ischemic stroke who took aspirin for secondary prevention, compared with wild-type patients, the ARU value of aspirin response unit in patients with GPⅠa(C807T) mutation group was significantly higher, and the number of patients with ARU≥550 was significantly higher than that in wild-...

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Abstract

The invention discloses a COX-1 gene polymorphism site related to aspirin resistance and application. Antiplatelet drugs (especially aspirin) play a crucial role in preventing and curing the ischemic stroke, however, current survey results show that even if many ischemic stroke patients have taken the antiplatelet drugs, the stroke still reoccurs repeatedly, and the phenomenon is called antiplatelet drug resistance. An applicant defines one COX-1 gene polymorphism site COX-1(A842G) related to aspirin resistance, and designs a primer detecting site polymorphism for the site, and the primer can be used for preparing kits screening the aspirin resistance patients so as to select the antiplatelet drugs for the ischemic stroke patients and reduce aspirin resistance and the stroke reoccurrence rate.

Description

technical field [0001] The invention relates to the field of drug selection for treating medical diseases, in particular to aspirin resistance-related COX-1 gene polymorphism sites and applications. technical background [0002] With the aging population in our country, the incidence of cerebrovascular disease is increasing year by year. The latest survey results show that cerebrovascular disease has become the number one killer of Chinese people beyond tumors since 2010. There are 2 million new cases in my country every year. Chronic cerebrovascular disease accounts for 70-80%, recurrent patients account for 27%, and 70% of patients have neurological deficits. The high mortality rate, disability rate and recurrence rate of cerebrovascular disease have caused a great social burden and economic burden. burden. Antiplatelet drugs (especially aspirin) play a vital role in the prevention and treatment of cerebrovascular diseases, but the current survey results show that many pati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 胡波毛玲夏远鹏贺权威靳慧娟李亚男
Owner 武汉欧瑞康安生物科技有限公司
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