Preparation method for paper-based micro-fluidic chip

A microfluidic chip, paper-based technology, applied in chemical instruments and methods, laboratory containers, material analysis by observing the effect of chemical indicators, etc., can solve the problems of inconvenient online detection and troublesome operation, etc. Achieve the effect of low production cost, short production cycle and good application prospects

Active Publication Date: 2015-10-28
XINJIANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the former needs to be heated at 100° for 35 minutes; while the latter needs to be sonicated in an ice bath for 1 hour, which brings some troubles to the operation and is not convenient for online detection

Method used

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  • Preparation method for paper-based micro-fluidic chip
  • Preparation method for paper-based micro-fluidic chip
  • Preparation method for paper-based micro-fluidic chip

Examples

Experimental program
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preparation example Construction

[0040] (1) Preparation steps of hydrophobic substrate:

[0041]First, dissolve trimethoxysilane in n-heptane or n-hexane with a purity of 99% at room temperature to prepare a hydrophobizing solution with a volume concentration of 2% to 6%; secondly, place whatman filter paper in the hydrophobizing solution at room temperature Soak in the water for 10 seconds; finally, take out the filter paper and dry it in the air for 1 minute to obtain a hydrophobic base;

[0042] (2) Preparation steps of hydrophilic substrate:

[0043] First, use CAD software to design the configuration of the hydrophilic channel required for the experiment, such as figure 2 As shown; secondly, use the printer to print the above-mentioned designed pattern on the filter paper, and cut the hydrophilic channel configuration pattern printed on the filter paper; finally, soak the cut hydrophilic pattern in a mass concentration of 0.8%~ 2% CTAB solution to obtain a hydrophilic substrate;

[0044] The hydrophi...

Embodiment 1

[0051] A method for preparing a hydrophobized substrate of a paper-based microfluidic chip for glucose concentration detection, comprising the following steps.

[0052] (1) 2 mL of trimethoxysilane (Aladdin Company) and 2.5 mL of ethyl acetate were dissolved in 50 mL of heptane at room temperature to obtain a hydrophobic solution;

[0053] (2) Soak the cut filter paper in the above hydrophobizing solution for 10 seconds at room temperature, take it out and dry it in the air for 1 minute to obtain the hydrophobizing substrate;

[0054] (3) Design the shape of the hydrophilic channel with CAD software, then print it on the filter paper, and finally cut out the hydrophilic channel with scissors according to the printed pattern;

[0055] (4) Weigh 1g of CTAB dissolved in 100mL of distilled water and stir until completely dissolved, and store in a refrigerator at 4°C in the dark, and mix 9mL of the above CTAB solution with 1mL of absolute ethanol to obtain a hydrophilic channel soa...

Embodiment 2

[0060] In order to prove that the solvent used in the present invention is easier to make and faster than the solvent in the prior art, the inventor has also done the following comparative experiments;

[0061] The difference from the preparation process of the hydrophobic substrate in Example 1 is that octadecyltrimethoxysilane (TMOS) is used to replace trimethoxysilane for the preparation of the hydrophobic substrate;

[0062] Soak the hydrophobic substrate prepared in Example 1 and the hydrophobic substrate prepared in Example 2 into a beaker filled with water, and observe the degree of hydrophobicity;

[0063] See Figure 4 , the filter paper treated with trimethoxysilane in Example 1 floats to the top of the water in the beaker, while the filter paper treated with TMOS sinks to the bottom of the beaker; the above phenomenon shows that the filter paper treated with trimethoxysilane can quickly make the hydrophilic Water filter paper becomes hydrophobic, while TMOS cannot ...

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Abstract

The invention discloses a preparation method for a paper-based micro-fluidic chip. The trimethoxy silane is processed in a heptane solution to obtain a hydrophobic base. Meanwhile, patterns printed on the channel of a piece of filter paper are cut, and then are immersed in cetyl trimethyl ammonium bromide to obtain a hydrophilic base. The above hydrophobic base and the above hydrophilic base are bonded together to form a hydrophilic and hydrophobic access. Compared with the traditional method, the above preparation method is free of any expensive instrument/agent/metal mold, thus being novel, simple, quick, low in cost and short in production period. The entire preparation process can be completed within 7 minutes.

Description

technical field [0001] The invention belongs to the field of chemical chip preparation, and in particular relates to a method for manufacturing a paper-based microfluidic chip. Background technique [0002] Paper chip (μPADs) is a paper-based microfluidic device developed in recent years. It has the advantages of low cost, simple operation, no need for external equipment, and multiple detection. It combines the advantages of commonly used microfluidic devices. (Inexpensive materials, small sample volume, high throughput) combined with the advantages of lateral flow detection (through capillary action to drive liquid flow, adsorb reagents, and filter samples, without the need for expensive pumps or external aids such as pressure control valves), so in It has great application prospects in a wide range of fields such as medical diagnosis, drug development, water quality testing and environmental quality monitoring. [0003] At present, technologies such as ultraviolet lithogr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78B01L3/00
Inventor 张道宏薛媛媛王建龙朱文新张文涛于少轩王帅星张玉环王靖
Owner XINJIANG AGRI UNIV
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