Probiotic preparation for preventing and treating vascular dementia and preparation method of probiotic preparation

A technology of vascular dementia and strains, applied in the field of biopharmaceuticals, can solve problems such as unreported, and achieve the effect of convenient use, good ecological mutual assistance, and improvement of intestinal flora imbalance

Inactive Publication Date: 2015-11-04
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, we speculate that there may be a certain relationship between intestinal flora and vasc...

Method used

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  • Probiotic preparation for preventing and treating vascular dementia and preparation method of probiotic preparation

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Isolation and identification of Clostridium butyricum (Clostridium butyricum) WZMC1016 bacterial strain

[0027] Using the feces of a healthy young man as an isolated sample, on the improved MRS agar medium, under anaerobic conditions at 37°C, smear, isolate and cultivate for 48 hours to obtain Clostridium butyricum WZMC1016 according to the present invention, and identify Clostridium butyricum.

[0028] The formula of the improved MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, K 2 HPO 4 2g, MgSO 4 7 hours 2 O 0.5g, MnSO 4 4H 2 O 0.2g, fructooligosaccharide 3g, diammonium citrate 2g, agar 15g, distilled water 1L, adjust the pH to 7.0, and sterilize at 115°C for 15 minutes.

[0029] Clostridium butyricum (Clostridium butyricum) WZMC1016 of the present invention has the following microbiological characteristics:

[0030] (1) Colony shape: milky white, round and slightly convex, with irregular edges, 1-3 mm in diameter, slightly...

Embodiment 2

[0035] Preparation of Example 2 Clostridium butyricum (Clostridium butyricum) WZMC1016 probiotic preparation composition

[0036] 1. Seed solution preparation: pick a single colony of Clostridium butyricum (Clostridium butyricum) WZMC1016, inoculate it in 50 mL of modified MRS liquid medium, pH 7.0±0.5, 37°C, anaerobic culture for 24 hours, that is the seed solution;

[0037] The formula of the improved MRS medium: peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium acetate 5g, K 2 HPO 4 2g, MgSO 4 7 hours 2 O 0.5g, MnSO 4 4H 2 O 0.2g, fructooligosaccharide 3g, diammonium citrate 2g, distilled water 1L, adjust the pH to 7.0, and sterilize at 115°C for 15min.

[0038] 2. Fermentation culture: Take the seed solution in step 1, inoculate it into a new improved MRS medium with an inoculum amount of 1%, culture it anaerobically at 37°C for 24 hours, and end the culture;

[0039] 3. Collect the bacteria: take the fermentation liquid in step 2, remove the ferm...

Embodiment 3

[0042] Therapeutic animal experiment of embodiment 3 vascular dementia

[0043] Objective: To observe the effect of the Clostridium butyricum preparation of the present invention on the learning and memory, the oxidation index in the brain and the morphological changes of nerve cells in mice with vascular dementia.

[0044] 1. Materials and methods:

[0045] 1. Drug: Clostridium butyricum (Clostridium butyricum) WZMC1016 bacteria powder, the number of viable bacteria is 1×10 8 CFU / g, the specific preparation method, see Example 2 for details.

[0046] 2. Experimental animals: male ICR mice, weighing 18-22 grams.

[0047] 3. Vascular dementia (VaD) mouse model preparation and animal grouping: permanent unilateral right common carotid artery occlusion (rUCCAO), mice were anesthetized, fixed on the operating table, separated and sutured with permanent small silk thread The right common carotid artery was performed, and the body temperature of the mice was kept at a constant te...

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Abstract

The invention belongs to the field of life science, relates to an application of a probiotic preparation in treating and preventing vascular dementia, in particular to a new application of medicine for preventing and treating vascular dementia through clostridium butyricum. Vascular dementia is brain tissue damage existing on the basis of multiple cerebrovascular diseases and the syndrome with cognition impairment as main clinical manifestation. The probiotic preparation for preventing and treating vascular dementia comprises medicine, healthcare products and drinks. No toxic or side effect is found. Intestinal flora is creatively adopted as treatment target points, a new pattern for preventing and treating vascular dementia is created, disease symptoms are relieved, nerve cell damage is retarded, good social and economic benefits are achieved, and the probiotic preparation is worthy of being applied and popularized.

Description

technical field [0001] The invention relates to a new application of Clostridium butyricum, in particular to the application of Clostridium butyricum in the preparation of a composition for preventing and treating vascular dementia, and belongs to the field of biopharmaceuticals. Background technique [0002] Vascular dementia (VaD) is a syndrome characterized by brain tissue damage on the basis of various cerebrovascular diseases and cognitive dysfunction as the main clinical manifestation. Studies have shown that VaD is the second largest dementia after Alzheimer's disease AD, accounting for about half of all dementia patients in my country. The prevalence of VaD in China is 1.1% to 3.0%. In 5-9 / 1000 people, and the incidence rate is increasing year by year, among stroke patients over 65 years old, about 25%-41% of stroke patients can develop VaD within three months. With the aging of society in our country, there will be more and more patients with cerebrovascular disease...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61P25/28A23L1/29A23L33/00
Inventor 刘佳明孙晶
Owner WENZHOU MEDICAL UNIV
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