Hybridoma cell line 7D7 secreting CAV-2 monoclonal antibody, monoclonal antibody secreted by hybridoma cell line 7D7, and applications of monoclonal antibody
A hybridoma cell line, CAV-2 technology, applied in the field of monoclonal antibodies, can solve the problem of weak virulence of canine adenovirus type 2
Inactive Publication Date: 2015-11-04
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology
Generally, the isolated canine adenovirus type 2 has weak virulence, and the relevant research mainly focuses on its application as a vector, while there are relatively few studies on its pathogenicity
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[0037] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention. within the scope of protection.
[0038] The invention provides a method for preparing anti-CAV-2 monoclonal antibodies that recognize different epitopes, the specific contents are as follows:
[0039] 1 Materials and methods
[0040] 1.1 Materials, experimental animals and reagents
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The present invention discloses a strain of a hybridoma cell line 7D7 secreting CAV-2 monoclonal antibody, monoclonal antibody secreted by the hybridoma cell line 7D7, and applications of the monoclonal antibody, wherein the preservation number of the hybridoma cell line 7D7 is CGMCC No.10877. According to the present invention, the indirect immunofluorescence results show that: the monoclonal antibody secreted by the hybridoma cell line 7D7 can specifically recognize CAV-2 virus and do not react with MDCK cells; the superposition experiment results of the prepared monoclonal antibody secreted by hybridoma cell line 7D7 and the monoclonal antibody secreted by 2C1 show that the two monoclonal antibodies are against different epitopes of the CAV-2 virus; the prepared monoclonal antibody can well react with CAV-2, and can be used for identification of clinical isolated strains; and the prepared 7D7 monoclonal antibody and the monoclonal antibody 2C1 are the monoclonal antibodies identifying the two different antigenic determinants of the CAV-2, such that the monoclonal antibody of the present invention can be used for preparation of the CAV-2 pathogenic derection immune colloidal gold test paper strip and establishment of the double antibody sandwich ELISA method.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a hybridoma cell line secreting CAV-2 monoclonal antibody, monoclonal antibodies secreted by the cell line to recognize different epitopes and applications thereof. Background technique [0002] Currently, the known serotypes of canine adenovirus are type 1 and type 2. In 1962, Dltehfleld isolated canine laryngotracheitis virus (CAV-2) from puppies suffering from respiratory diseases. Related, but distinctly different. [0003] Ditchfleld et al. have demonstrated the difference between CAV-1 and CAV-2 in terms of virulence, solubility, antigen structure and erythrocyte agglutination range. However, type 1 adenovirus can also cause simple respiratory diseases, so CAV-2 can be considered as a variant of CAV-1, and dogs immunized with CAV-2 can effectively produce strong immunity against CAV-1. So far, many strains of dog adenovirus type 1 and type 2 have been isolated one after anothe...
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IPC IPC(8): C12N5/20C07K16/08G01N33/577G01N33/569
Inventor 王淑杰蔡雪辉
Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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