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Method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp
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A soluble, organic acid technology, applied in the field of chemical analysis and instrumental analysis in the chemical industry, can solve problems such as sample damage, harsh conditions, and slow down research progress, and achieve the effect of slowing down degradation and saving materials
Inactive Publication Date: 2015-11-04
JIANGSU ACAD OF AGRI SCI
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Also have a few document reports to utilize pure water or the ethanol aqueous solution of certain concentration (mostly being 80% ethanol) to extract soluble sugar and organic acid simultaneously (as document " 1-MCP is to the influence of sugar-acid component and content of Kate apricot fruit postharvest " ; "Analysis of the Composition and Content of Sugar and Acid in Different Varieties of Apple Fruits", etc.), the method of direct extraction or ice bath or hot bath extraction is chosen, the operation is complicated and the conditions are harsh, and all are carried out under neutral conditions. There are literatures to adjust the pH by adding acidic substances to the extractant during simultaneous extraction
[0003] Considering that in practice, when a large number of test samples are separated and measured, the extraction of soluble sugars and organic acids is not only a waste of materials, but also time-consuming and labor-intensive; the extraction conditions in ice bath or hot bath are also relatively harsh, so if it can be realized in Simultaneous and efficient extraction at room temperature is the best strategy; but so far, no literature has proposed a method that can simultaneously and efficiently extract soluble sugars and organic acids in fruits
[0004] In addition, in practice, when conventional pure water or ethanol aqueous solution is used as the extractant, especially when pure water is used as the extractant, after adding the extractant, the degradation or fermentation of sucrose will occur in less than a week at room temperature. Destruction, seriously affecting the conduct of follow-up experiments, slowing down the progress of research
At present, this problem can only be avoided by freezing storage at -20°C or speeding up the determination. However, when the sample volume is too large, these processing methods cannot meet the actual needs.
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Embodiment 1
[0041] a. Accurately weigh 4g of metaphosphoric acid, dissolve and constant volume with 1L ultrapure water to obtain a 0.4% metaphosphoric acid aqueous solution; pipette 800ml absolute ethanol and 200ml 0.4% metaphosphoric acid aqueous solution respectively, and mix to obtain extractant ethanol: 0.4 % metaphosphoric acid aqueous solution=80:20V / V;
[0042] b. Accurately weigh 0.5g of ripe fruit pulp of the 'Xiahui No. 8' variety, add 8mL extractant, ultrasonically extract for 1h, centrifuge at 10,000rpm at 4°C for 10min, take the supernatant and filter it through a 0.22mm water filter head to obtain a sample solution;
[0043] c, under chromatographic conditions, sample solution is carried out to the high-performance liquid chromatography analysis of soluble sugar and organic acid respectively, obtain as follows figure 1 The HPLC profile of the soluble sugars shown, and as figure 2 HPLC profiles of the indicated organic acids. The calculated contents of various organic acid...
Embodiment 2
[0061] a. Accurately weigh 4g of metaphosphoric acid, dissolve it with 1L of ultrapure water to obtain a 0.4% metaphosphoric acid aqueous solution; respectively pipette 800ml of absolute ethanol and 200ml of 0.4% metaphosphoric acid aqueous solution, and mix to obtain the extractant ethanol: 0.4% metaphosphoric acid aqueous solution = 80:20.
[0062] b. Accurately weigh 0.5g of 4 portions of ripe fruit pulp of the 'Xiahui No. 8' variety, add 8mL extractant ethanol:0.4% metaphosphoric acid aqueous solution=80:20V / V, and let stand at room temperature. After standing for 1 week, 2 weeks, 3 weeks and 4 weeks respectively, centrifuge at 10,000 rpm for 10 min at 4°C, take the supernatant and filter it through a 0.22 mm water filter head to obtain a sample solution.
[0063] c. Under the same chromatographic conditions as in Example 1, the high performance liquid chromatography analysis of soluble sugar is carried out to the sample solution respectively, and the following results are o...
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Abstract
The invention discloses a method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp. The method comprises, a, preparing an extraction agent according to a ratio of ethanol to 0.4% metaphosphoric acid aqueous solution of 80: 20V / V (pH of 4.1), and b, weighing peach pulp, adding the extraction agent into the peach pulp according to a material-liquid ratio of 1: 16, carrying out ultrasonic-assisted extraction for 1h, carrying out centrifugation, taking a supernatant, carrying out condensation by a condensation device until drying, and dissolving the extract in ultrapure water to obtain the soluble sugar and organic acid solution. The method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp utilizes the extraction agent comprising ethanol and 0.4% metaphosphoric acid aqueous solution according to a ratio of 80: 20V / V, is free of additional condition of ice bath or heat bath, and realizes efficient and comprehensive extraction of soluble sugar and organic acid from peach pulp at a normal temperature. The extraction agent can delay extraction solution cane sugar degradation, does not produce obvious degradation conversion in four weeks, does not influence later experiment result reliability, and greatly saves materials, time and a cost.
Description
technical field [0001] The invention relates to a method for simultaneously extracting soluble sugar and organic acid in peach pulp, belonging to the technical field of chemical analysis and instrument analysis in the chemical industry. Background technique [0002] In practice, when the soluble sugar and organic acid of the fruit are separated and measured, the soluble sugar and organic acid are basically extracted separately, and they are basically extracted by pure water or a certain concentration of ethanol or methanol aqueous solution. The extraction conditions are all heating conditions, that is, the water bath ranges from 75-100°C. Also have a few document reports to utilize pure water or the ethanol aqueous solution of certain concentration (mostly being 80% ethanol) to extract soluble sugar and organic acid simultaneously (as document " 1-MCP is to the influence of sugar-acid component and content of Kate apricot fruit postharvest " ; "Analysis of the Composition a...
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