Method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp

Abstract

The invention discloses a method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp. The method comprises, a, preparing an extraction agent according to a ratio of ethanol to 0.4% metaphosphoric acid aqueous solution of 80: 20V / V (pH of 4.1), and b, weighing peach pulp, adding the extraction agent into the peach pulp according to a material-liquid ratio of 1: 16, carrying out ultrasonic-assisted extraction for 1h, carrying out centrifugation, taking a supernatant, carrying out condensation by a condensation device until drying, and dissolving the extract in ultrapure water to obtain the soluble sugar and organic acid solution. The method for simultaneously and efficiently extracting soluble sugar and organic acid from peach pulp utilizes the extraction agent comprising ethanol and 0.4% metaphosphoric acid aqueous solution according to a ratio of 80: 20V / V, is free of additional condition of ice bath or heat bath, and realizes efficient and comprehensive extraction of soluble sugar and organic acid from peach pulp at a normal temperature. The extraction agent can delay extraction solution cane sugar degradation, does not produce obvious degradation conversion in four weeks, does not influence later experiment result reliability, and greatly saves materials, time and a cost.

Description

technical field [0001] The invention relates to a method for simultaneously extracting soluble sugar and organic acid in peach pulp, belonging to the technical field of chemical analysis and instrument analysis in the chemical industry. Background technique [0002] In practice, when the soluble sugar and organic acid of the fruit are separated and measured, the soluble sugar and organic acid are basically extracted separately, and they are basically extracted by pure water or a certain concentration of ethanol or methanol aqueous solution. The extraction conditions are all heating conditions, that is, the water bath ranges from 75-100°C. Also have a few document reports to utilize pure water or the ethanol aqueous solution of certain concentration (mostly being 80% ethanol) to extract soluble sugar and organic acid simultaneously (as document " 1-MCP is to the influence of sugar-acid component and content of Kate apricot fruit postharvest " ; "Analysis of the Composition a...

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Problems solved by technology

Also have a few document reports to utilize pure water or the ethanol aqueous solution of certain concentration (mostly being 80% ethanol) to extract soluble sugar and organic acid simultaneously (as document " 1-MCP is to the influence of sugar-acid component and content of Kate apricot fruit postharvest " ; "Analysis of the Composition and Content of Sugar and Acid in Different Varieties of Apple Fruits", etc.), the method of direct extraction or ice bath or hot bath extraction is chosen, the operation is complicated and the conditions are harsh, and all are carried out under neutral conditions. There are literatures to adjust the pH by adding acidic substances to the extractant during simultaneous extraction

[0003] Considering that in practice, when a large number of test samples are separated and measured, the extraction of soluble sugars and organic acids is not only a waste of materials, but also time-consuming and labor-intensive; the extraction conditions in ice bath or hot bath are also relatively harsh, so if it can be realized in Simultaneous and efficient extraction at room temperature is the best strategy; but so far, no literature has proposed a method that can simultaneously and efficiently extract soluble sugars and organic acids in fruits

[0004] In addition, in practice, when conventional pure water or ethanol aqueous solution is used as the extractant, especially when pure water is used as the extractant, after adding the extractant, the degradation or fermentation of sucrose will occur in less than a week at room temperature. Destruction, seriously affecting the conduct of follow-up experiments, slowing down the progress of research

At present, this problem can only be avoided by freezing storage at -20°C or speeding up the determination. However, when the sample volume is too large, these processing methods cannot meet the actual needs.

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