Composition and preparing method and beauty preparation thereof

A composition and preparation technology, applied in the field of cosmetic plastic surgery, can solve the problems of single composition of cosmetic preparations and insufficient effect, and achieve the effects of improving wrinkle-removing function, diluting scars and stains, and promoting angiogenesis.

Inactive Publication Date: 2015-11-11
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the existing cosmetic preparations have rel...

Method used

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  • Composition and preparing method and beauty preparation thereof
  • Composition and preparing method and beauty preparation thereof
  • Composition and preparing method and beauty preparation thereof

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preparation example Construction

[0037] The present invention also provides a preparation method of the above composition, comprising: mixing 30-70 parts by volume of cell growth factor solution, 30-70 parts by volume of high-concentration platelet plasma and 0.1×10 6 ~2×10 6 Adipose stem cells / mL were mixed to obtain a composition.

[0038] Wherein, the cell growth factor solution, high-concentration platelet plasma, and adipose stem cells are all the same as above, and will not be repeated here. The cell growth factor solution is obtained by mixing the freeze-dried cell growth factor powder with a solvent, and the solvent is a solvent well-known to those skilled in the art, and there is no special limitation. In the present invention, physiological saline is preferably used as the solvent.

[0039] According to the present invention, it is preferred to mix 30 to 70 parts by volume of high-concentration platelet plasma with 0.1×10 6 ~2×10 6 Adipose stem cells per mL are mixed, and then mixed with 30-70 ...

Embodiment 1

[0046] 1.1 Take the adipose stem cells of the P1 generation with a confluence of 90%, remove the culture supernatant, wash with normal saline for 3 times, and add 0.02mL / cm 2 0.25% trypsin and 0.02% EDTA were digested for 2 minutes, and the complete medium (lonza UltraCULTUREMEDIUM serum-free medium + PALL serum substitute + 2mML-glutamine + 1xNEAA) 10 times that of the digestion solution was used to terminate the enzymolysis, and the samples were counted. Centrifuge at 200g for 5min, remove the supernatant, resuspend the cells with normal saline, adjust the total number of cells to 5×10 according to the counting results 6 Add normal saline to 45mL, centrifuge at 200g for 5min, wash the cells twice according to the above steps, and finally precipitate the prepared adipose stem cells.

[0047] 1.2 Take 20 mL of autologous whole blood and centrifuge at 400 g for 10 min, take plasma, discard red blood cells, centrifuge at 1200 g for 5 min, keep 2 mL of plasma, discard the rest,...

Embodiment 2

[0050] 2.1 Take the adipose stem cells of the P2 generation with a confluence of 80%, remove the culture supernatant, wash with normal saline for 3 times, and add 0.02mL / cm 2 0.25% trypsin and 0.02% EDTA were digested for 2 minutes, and the complete medium (lonza UltraCULTUREMEDIUM serum-free medium + PALL serum substitute + 2mML-glutamine + 1xNEAA) 10 times that of the digestion solution was used to terminate the enzymolysis, and the samples were counted. Centrifuge at 200g for 5min, remove the supernatant, resuspend the cells with normal saline, and adjust the total number of cells to 0.5×10 according to the counting results. 6 Add normal saline to 45mLmL, centrifuge at 200g for 5min, wash the cells twice according to the above steps, and finally precipitate the prepared adipose stem cells.

[0051] 2.2 Take 20mL of autologous whole blood and centrifuge at 400g for 10min, get the plasma, discard the red blood cells, centrifuge at 1200g for 5min, keep 2.5mL of plasma, disca...

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Abstract

The invention provides a composition and a preparing method and beauty preparation thereof. The composition comprises 30-70 volume parts of cell growth factor solutions, 30-70 volume parts of high-concentration blood platelet plasma, and adipose-derived stem cells with the concentration of 0.1*10<6>-2*10<6> /mL. Compared with the prior art, the composition is used for the beauty preparation, and the cell growth factor is a high-activity factor capable of promoting cell growth and promoting angiogenesis, wound healing and tissue damage repairing; the high-concentration blood platelet plasma is beneficial for binding cells and cell surface receptors and improving release of extracellular matrix protein and has a remarkable effect on removing wrinkles and relieving scars and stains; the adipose-derived stem cells can secrete various activity factors with remarkable biological functions, and the three substances interact to improve the wrinkle removal function of the beauty preparation.

Description

technical field [0001] The invention belongs to the technical field of cosmetic plastic surgery, and in particular relates to a composition, a preparation method thereof, and a cosmetic preparation. Background technique [0002] Facial aging is the result of changes in the body and the soft and hard tissues of the face and neck. Facial aging is closely related to factors such as physical fitness, genetic factors, living environment, psychological emotions, nature of work, nutritional status, diseases, and local skin care. After the average person reaches the age of 25, signs of aging begin to appear, and the formation of facial skin wrinkles is an important feature and indicator. [0003] Facial rejuvenation refers to various means and methods (including shape, anatomy and physiological structure, etc.) that can restore the aging face to a youthful appearance. With the improvement of living standards, people have higher and higher requirements for temperament and appearanc...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/64A61Q19/00A61Q19/02A61Q19/08
Inventor 王一飞陈海佳葛啸虎麦锦连马岩岩
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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