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C-kit gene polymorphism detection primer system and kit thereof

A technology for gene polymorphism and primer detection, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of time-consuming, labor-intensive and expensive, and achieve a wide range of applications, wide application range, Detect quick and easy effects

Inactive Publication Date: 2015-11-11
上海赛安生物医药科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Time-consuming, labor-intensive and costly, there is no effective and economical detection product at present

Method used

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  • C-kit gene polymorphism detection primer system and kit thereof
  • C-kit gene polymorphism detection primer system and kit thereof
  • C-kit gene polymorphism detection primer system and kit thereof

Examples

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Embodiment 1

[0030] 1. The composition of the kit.

[0031] The c-kit gene polymorphism detection kit of this embodiment includes: a reaction solution A for detecting that the c.2446 site of the c-kit gene is G, and for detecting that the c.2446 site of the c-kit gene is T Reaction solution B for the case where the c-kit gene c.2446 site is C. Reaction solution C for the case where the c-kit gene c.2447 site is A. Reaction solution D for the case where c-kit gene c.2447 site is A -Reaction solution E for the case where the c.2447 site of the kit gene is T, and reaction solution F for detecting the β-actin gene as an internal control.

[0032] The preparation of reaction solution A, B, C, D, E, F is as follows:

[0033] 1. Preparation of reaction solution A (MIXA).

[0034] Reaction solution A is the reaction mixture for detecting c-kit gene c.2446 site, and the designed primers are aimed at the situation that c-kit gene c.2446 site is G, including the c-kit gene c.2446 site being Forwar...

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Abstract

The invention relates to a c-kit gene polymorphism detection primer. The c-kit gene polymorphism detection primer comprises a forward primer producing the G situation for the c.2446 locus of a c-kit gene, a forward primer producing the C situation for the c.2446 locus of the c-kit gene, a forward primer producing the T situation for the c.2446 locus of the c-kit gene, a forward primer producing the A situation for the c.2447 locus of the c-kit gene, a forward primer producing the T situation for the c.2447 locus of the c-kit gene, a universal reverse primer corresponding to the c.2446 locus and c.2447 locus of the c-kit gene, a forward primer for detecting a beta-acctin gene and a reverse primer for detecting the beta-acctin gene. The c-kit gene polymorphism detection kit enables detection to be quick and convenient and is high in accuracy rate and low in cost.

Description

technical field [0001] The invention relates to a gene mutation detection product, a detection primer and a detection system used in the product, and belongs to the field of biotechnology. Background technique [0002] The c-kit gene is located on human chromosome 4q12-13 and belongs to the proto-oncogene. Its product is type III tyrosine kinase, which is one of the important members of the tyrosine kinase receptor protein family. As a receptor for stem cell factors, it can Participate in the regulation of hematopoietic stem cell proliferation and differentiation through a series of signaling pathways. In recent years, studies have found that there are mutations in the c-kit gene, and the mutations are mainly in the 9th, 11th, 13th, and 17th exons. The mutation of c-kit gene is closely related to the pathogenesis, treatment and prognosis of gastrointestinal stromal tumor (GIST) and acute myeloid leukemia (AML). Studies have also confirmed the secondary mutation of the c-ki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6858C12Q1/6886C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 赵新泰王明唐娟娟张红霞
Owner 上海赛安生物医药科技股份有限公司
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