Plant seed specific expression promoter ossee1

A seed and expression vector technology, applied in the fields of biotechnology and plant genetic engineering, can solve problems such as inability to meet the needs of research and production, insufficient research, etc., and achieve the effects of increasing content, increasing development speed, and improving taste

Inactive Publication Date: 2018-08-28
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is obvious that the current research on seed-specific promoters is not enough, and the currently disclosed seed-specific promoters cannot meet the needs of research and production.

Method used

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  • Plant seed specific expression promoter ossee1
  • Plant seed specific expression promoter ossee1
  • Plant seed specific expression promoter ossee1

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Embodiment Construction

[0034] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0035] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0036] Acquisition of OsSee1 promoter containing restriction site

[0037] Step 1. Design of primers

[0038] According to the whole genome sequence of rice variety Nipponbare (Oryza sativa L cv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsSee1 gene, and the enzyme cutting sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0039...

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Abstract

The invention discloses the cloning and application of a plant seed specific expression promoter OsSee1. The invention also provides an expression box and a plant expression vector containing the promoter and its application in plant genetic engineering. The inventors of the present application obtained and verified a DNA sequence with special functions—the rice seed-specific expression promoter OsSee1—by amplifying, isolating, analyzing, utilizing, and experimentally verifying the specific genome of the rice variety Nipponbare. The promoter provided by the present invention can specifically drive the expression of exogenous genes in rice seeds. Therefore, in genetic engineering, the promoter can be used to replace the constitutive promoter to drive the target gene, which can specifically express the target gene and avoid other factors. Ineffective expression of genes can improve rice quality.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice seed-specific expression promoter OsSee1 and its application. The promoter can specifically drive the expression of a target gene in seeds in a rice transgenic regulation system. Background technique [0002] Plant gene expression is regulated and controlled by various physical and chemical factors in the organism. Plants can express the required gene products in a timely, localized and quantitative manner according to their own needs and environmental changes. Gene expression is finely regulated at all levels, such as DNA level, transcription level, post-transcription level, translation level, post-translational processing, transport and localization. Gene transcription level expression regulation is the most important regulation method in gene expression regulation. In this series of processes, regulator...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12N15/84C12N1/21A01H5/00A01H6/46
Inventor 魏鹏程杨剑波李莉李浩许蓉芳马卉杨亚春秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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