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Plant seed specific expression promotor OsSee1

A promoter and specific technology, applied in the fields of biotechnology and plant genetic engineering, can solve problems such as insufficient research and inability to meet the needs of research and production, and achieve the effects of increasing development speed, increasing content, and improving taste

Inactive Publication Date: 2015-11-18
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is obvious that the current research on seed-specific promoters is not enough, and the currently disclosed seed-specific promoters cannot meet the needs of research and production.

Method used

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  • Plant seed specific expression promotor OsSee1
  • Plant seed specific expression promotor OsSee1
  • Plant seed specific expression promotor OsSee1

Examples

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Embodiment Construction

[0034] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only used to illustrate the present invention and do not limit the scope of the present invention in any way.

[0035] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.

[0036] Acquisition of OsSee1 promoter containing restriction site

[0037] Step 1. Design of primers

[0038] According to the whole genome sequence of the rice variety Nipponbare (Oryzasativa Lcv. Nipponbare) provided by NCBI, the amplification primers were designed according to the sequence of the rice OsSee1 gene, and the restriction sites of the primers were designed according to the characteristics of the selected vector and target gene.

[0039]...

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Abstract

The invention discloses clone and application of a plant seed specific expression promotor OsSee1, and further provides an expression cassette comprising the promoter, a plant expression vector and application of the plant seed specific expression promotor OsSee1 to plant genetic engineering. Through the amplification, separation, analysis, utilization and experimental verification of special genomes of Oryza sativa L cv.Nipponbare by an inventor, a section of DNA sequence, namely the rice seed specific expression promotor OsSee1, with a special function is obtained, and the special function is verified. The promotor provided by the invention can specifically drive the expression of exogenous genes in rice seeds, so that in the gene engineering, when the promotor is used for replacing a constitutive promoter to drive a target gene, not only is the target gene specifically expressed, but also invalid expression of other genes is avoided, and the effect of improving the rice quality is realized.

Description

technical field [0001] The invention relates to the technical fields of biotechnology and plant genetic engineering. Specifically, the present invention relates to a rice seed-specific expression promoter OsSee1 and its application. The promoter can specifically drive the expression of a target gene in seeds in a rice transgenic regulation system. Background technique [0002] Plant gene expression is regulated and controlled by various physical and chemical factors in the organism. Plants can express the required gene products in a timely, localized and quantitative manner according to their own needs and environmental changes. Gene expression is finely regulated at all levels, such as DNA level, transcription level, post-transcription level, translation level, post-translational processing, transport and localization. Gene transcription level expression regulation is the most important regulation method in gene expression regulation. In this series of processes, regulator...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12N15/11C12N15/84C12N1/21A01H5/00
Inventor 魏鹏程杨剑波李莉李浩许蓉芳马卉杨亚春秦瑞英
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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