Plant seed specific expression promotor OsSee1
A promoter and specific technology, applied in the fields of biotechnology and plant genetic engineering, can solve problems such as insufficient research and inability to meet the needs of research and production, and achieve the effects of increasing development speed, increasing content, and improving taste
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[0034] The present invention will be described below with reference to specific examples. Those skilled in the art can understand that these examples are only for illustrating the present invention, and they do not limit the scope of the present invention in any way.
[0035] The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal raw materials, reagent materials, etc. used in the following examples are all commercially available products unless otherwise specified.
[0036] Obtaining the OsSee1 Promoter Containing Restriction Site
[0037] Step 1. Design of primers
[0038] According to the whole genome sequence of rice variety Nipponbare (Oryzasativa Lcv. Nipponbare) provided in NCBI, the amplification primers were designed according to the sequence of rice OsSee1 gene, and the restriction sites of the primers were designed according to the characteristics of the selected vector and target gene.
[0039] In t...
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