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A method for identifying non-reconstituted fruit juice

A technology for fruit juice and raw juice, applied in the field of application research of stable isotope technology, can solve problems such as difficult operation, difficult effective application, poor measurement stability, etc., and achieve the effect of improving detection limit, avoiding interference and simple operation.

Active Publication Date: 2018-02-06
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Theoretically δ in sugar, citric acid or cellulose 18 O is used as an endogenous indicator of water for NFC fruit juice identification. However, due to the presence of a large amount of water in fruit juice, the analysis of soluble solids such as sugar and citric acid and δ in cellulose 18 O is easily disturbed by water [Jamin, E.; Guerin, R.; Retif, M.; Lees, M.; Martin, G.J. Improved detection of added water in orange juice by simultaneous determination of the oxygen-18 / oxygen-16isotope ratios of water and ethanol derived from sugars.J.Agric.Food Chem.2003,51,5202-5206], the operation is difficult and the measurement stability is poor, so the sugar, citric acid or cellulose is difficult to be effectively used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Determination of stable oxygen isotope composition δ in water of NFC orange juice A, B, C and orange juice a and b to be tested 18 o 橙汁水 (δ in water 18 The determination of O is carried out according to patent ZL201210473455.6), and the results are shown in Table 1.

[0041] Table 1 δ in water of orange juice 18 O determination result

[0042] juice

A

B

C

a

b

δ 18 o 水测定值 (‰)

9.42

-3.43

2.17

1.85

0.21

[0043] It can be seen from Table 1 that the δ in water of the three NFC orange juices A, B, and C 18 The difference in O is obvious, which shows that it is impossible to set a fixed decision limit for NFC orange juice, in which the orange juice a and b to be tested are both between orange juice B and C, and according to the judgment standard of AIJN for orange juice, juice a and b All are NFC juices.

[0044] 2. Weigh 200g each of A, B, and C orange juices into a 500mL Erlenmeyer flask, add 1% (m / m) CM796 bre...

Embodiment 2

[0057] In order to verify the accuracy and effectiveness of the present invention in identifying non-reconstituted fruit juices, samples mixed with different waters were prepared with orange juice A and tap water as research objects (see Table 4). Process each sample according to the method of the present invention, measure δ in water 18 O, ethanol δ 18 o 发酵乙醇 , and judged according to the model, the results are shown in Table 4.

[0058] Table 4 Data δ of simulated samples 18 O(‰)

[0059] Sample serial number

[0060] It can be seen from Table 4 that according to the orange juice judgment standard given by AIJN, even if orange juice A is mixed with 50% tap water, the water δ 18 O is still compliant. In the present embodiment, according to the identification model of the present invention, it can be seen that when the mixing ratio of tap water exceeds 15%, the water δ 18 The difference between the calculated value and the measured value of O has exceeded 1‰, s...

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Abstract

The invention relates to a method for identifying non-reconstituted fruit juice. Specifically, it is a method for identifying the authenticity of food based on stable isotope technology. Specifically, the method of the present invention is to construct a mathematical model of the stable oxygen isotope composition (denoted as δ18O) in the ethanol produced by the water of NFC fruit juice and the juice after dehydration fermentation, and the water of the product to be tested and its fermentation under specific conditions The δ18O measured value of the produced ethanol was substituted into the mathematical model for authenticity identification. The invention performs identification according to the δ18O characteristics of the fruit juice itself, does not need to establish a huge database, simplifies the identification steps, improves the detection limit, and is very suitable for authenticity identification of NFC fruit juices.

Description

technical field [0001] The invention relates to a method for identifying non-reconstituted fruit juice. Specifically, it belongs to the field of applied research of stable isotope technology, specifically according to the δ of fruit juice itself 18 O feature to identify the authenticity of NFC juice. Background technique [0002] Non-concentration (Not From Concentration, NFC) juice is a product that washes fresh raw fruit, squeezes out the juice, and directly fills it after instant sterilization. Its quality is close to natural freshly squeezed juice, and the price is 2-4 times, the added value is higher. NFC juice meets the trend of people's consumption demand for healthy beverages, and the market has great potential for development. Compared with the complex production process and strict storage conditions of NFC juice, reconstituted juice has an incomparable cost advantage. However, the content of each physical and chemical component in the non-reconstituted fruit ju...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/14
Inventor 钟其顶王道兵
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD