A kind of method that high performance liquid chromatography detects 3-amino-2-caprolactam
A high-performance liquid chromatography and caprolactam technology, which is applied in the field of high-performance liquid chromatography for detecting 3-amino-2-caprolactam, can solve problems such as narrow application range, and achieves good specificity, good linear fitting and good durability. Effect
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Embodiment 1
[0036] Embodiment 1: a kind of method that high performance liquid chromatography detects 3-amino-2-caprolactam, it comprises the following steps:
[0037] S1. Mobile phase preparation:
[0038] (1) Weigh 5.44 g of potassium dihydrogen phosphate, add water and dilute to 4000 ml to obtain a 0.01mol / L potassium dihydrogen phosphate solution, adjust the pH to 2 with phosphoric acid, and obtain mobile phase A after suction filtration and ultrasound;
[0039] (2) Prepare a solution with a volume ratio of methanol, acetonitrile and water of 1:5:1, shake well to obtain mobile phase B;
[0040] S2. Reference substance solution preparation:
[0041] (1) Weigh 10 mg of 3-amino-2-caprolactam and add it to a 50ml volumetric flask, dissolve it with mobile phase A and adjust the volume to the volumetric line, shake well to obtain 3-amino-2-caprolactam reference substance stock solution;
[0042] (2) Measure 1ml of 3-amino-2-caprolactam reference substance stock solution and add it to a 10...
Embodiment 2
[0045] Embodiment 2: a kind of method that high performance liquid chromatography detects 3-amino-2-caprolactam, it comprises the following steps:
[0046] S1. Mobile phase preparation:
[0047] (1) Weigh potassium dihydrogen phosphate 16.33g, add water and dilute to 4000ml to obtain 0.03mol / L potassium dihydrogen phosphate solution, adjust pH to 2.5 with phosphoric acid, obtain mobile phase A after suction filtration and ultrasonic;
[0048] (2) Prepare methanol, acetonitrile and water with a volume ratio of 2:4:1, shake well to obtain mobile phase B;
[0049] S2. Reference substance solution preparation:
[0050] (1) Weigh 20 mg of 3-amino-2-caprolactam and add it to a 50ml volumetric flask, dissolve it with mobile phase A and adjust the volume to the scale line of the volumetric flask, shake well to obtain 3-amino-2-caprolactam reference substance stock solution;
[0051] (2) Measure 1ml of 3-amino-2-caprolactam reference substance stock solution and add it to a 100ml vol...
Embodiment 3
[0054] Embodiment 3: a kind of method that high performance liquid chromatography detects 3-amino-2-caprolactam, it comprises the following steps:
[0055] S1. Mobile phase preparation:
[0056] (1) Weigh 27.22 g of potassium dihydrogen phosphate, add water and dilute to 4000 ml to obtain a 0.05 mol / L potassium dihydrogen phosphate solution, adjust the pH to 3 with phosphoric acid, obtain mobile phase A after suction filtration and ultrasound;
[0057] (2) Prepare a solution with a volume ratio of methanol, acetonitrile and water of 3:3:1, shake well to obtain mobile phase B;
[0058] S2. Reference substance solution preparation:
[0059] (1) Weigh 15 mg of 3-amino-2-caprolactam and add it to a 50ml volumetric flask, dissolve it with mobile phase A and adjust the volume to the scale line of the volumetric flask, shake well to obtain 3-amino-2-caprolactam reference substance stock solution;
[0060] (2) Measure 1ml of 3-amino-2-caprolactam reference substance stock solution a...
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