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Method of Tissue Culture and Rapid Propagation of Atractylodes Atractylodis

A tissue culture and fast technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of low utilization rate of clustered buds, high production cost, low work efficiency, etc., and reduce the risk of transferring test-tube seedlings, Low production cost and the effect of reducing the cost of seedling production

Inactive Publication Date: 2017-05-10
CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI
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  • Application Information

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Problems solved by technology

[0004] 1. The preparation and sterilization of each medium requires a lot of work, high production costs and low work efficiency
[0005] 2. Every time the cultivation is replaced, due to the need for manual operation on the ultra-clean bench, even experienced technical workers will cause a certain degree of pollution and waste, and the utilization rate of clustered buds is low
[0006] 3. In terms of the multiplication rate of cluster buds, 800% obtained by scientific researchers represented by Chao Jianguo is the highest, and the multiplication rate is relatively low

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  • Method of Tissue Culture and Rapid Propagation of Atractylodes Atractylodis
  • Method of Tissue Culture and Rapid Propagation of Atractylodes Atractylodis

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Embodiment Construction

[0029] Specific embodiments of the present invention will be described in detail below.

[0030] A method for tissue culture and rapid propagation of Atractylodes atractylodis, comprising the steps of:

[0031] (1) Select large and plump seeds, soak them in detergent water for 10 minutes, wash them with clean water and soak them in clean water for about 3-4 hours, then disinfect the surface with 75% alcohol for 30 seconds, and then use 0.1% mercuric chloride (mercuric chloride) Disinfect for 10 minutes, rinse with sterile water 4-5 times, inoculate on 1 / 2MS (sugar-containing 30g / L) basic medium, cultivate for two weeks, and obtain sterile vaccines.

[0032] (2) On 1 / 2MS (sugar 30g / L) + 6-BA 2mg / l + KT 1mg / l +NAA 0.5mg / l + GA 0.5mg / l culture medium, take the root and hypocotyl as Explant culture, PH=6.0±0.5, culture temperature 28°C-30°C, light intensity 2500Lx-2800Lx, light time 8 hours / day, proved that it is easier to form a complete plant with the hypocotyl as the explant. ...

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Abstract

The invention relates to the technical field of tissue culture and planting of plants, in particular to a tissue culture and rapid propagation method of Atractylodes chinensis. A conventional tissue culture method is improved and simplified; according to tissue culture and rapid propagation of the Atractylodes chinensis, gibberellin is added during inducing of cluster buds, so that the propagation rate is increased by multiple times, further, a rooting mode is simplified, natural rooting can be realized without addition of hormone for inducing rooting, accordingly, the propagation process is simplified, and the influence on the quality of the Atractylodes chinensis due to addition of hormone is avoided.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture and planting, in particular to a method for tissue culture and rapid propagation of Atractylodes atractylodis. Background technique [0002] Atractylodes atractylodis is one of the commonly used traditional Chinese medicines. It is in great demand and has the effects of expelling wind and cold, drying dampness and invigorating the spleen, and improving eyesight. Cangzhu can be divided into Northern Cangzhu and Southern Cangzhu (Mao Cangzhu). Northern herb A.chinensis (DC.)Koidz , is a perennial herb of Compositae, and its rhizome is used as medicine. Mainly distributed in Heilongjiang, Jilin, Liaoning, Inner Mongolia, Hebei, Shanxi, Shaanxi, Gansu, Ningxia, Qinghai and other provinces and autonomous regions. In the Zhongtiao Mountain and Taihang Mountains in Shanxi Province, there are excellent resources of Atractylodes maxima, which are endangered plants. In order to save this en...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 左静静刘少翔闫贵云王卉霍利光高红牛瑜琦安晓宁
Owner CROP SCI RES INST SHANXI ACADEMY OF AGRI SCI