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A kind of one-component TMB chromogenic solution and preparation method thereof

A chromogenic liquid, single-component technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as product instability, achieve the effects of eliminating ion interference, reducing damage, and promoting dissolution

Active Publication Date: 2016-09-14
SHENZHEN WEIGUANG BIOLOGICAL PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The concentration of hydrogen peroxide generally corresponds to the content of TMB, too much will cause a great increase in the background, resulting in unstable products

Method used

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  • A kind of one-component TMB chromogenic solution and preparation method thereof
  • A kind of one-component TMB chromogenic solution and preparation method thereof
  • A kind of one-component TMB chromogenic solution and preparation method thereof

Examples

Experimental program
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preparation example Construction

[0041] The present invention also provides a preparation method for the above-mentioned one-component TMB chromogenic solution, comprising the steps of:

[0042] (1) Solution A: Take 900ml of citric acid-sodium citrate buffer solution, add 0.188-1.88g of carbamide peroxide, stir to dissolve it fully, dilute to 1L with citric acid-sodium citrate buffer solution, and seal it temporarily in the dark. live.

[0043] (2) Liquid B: Weigh 4-10g of polyvinyl alcohol, add it to 500ml of 0.1mol / L citric acid-sodium citrate buffer solution at room temperature (not exceeding 40°C) while stirring, disperse evenly and then heat up Accelerate the dissolution, and finally stir and dissolve in a 60-65°C water bath, and keep warm for 2-2.5 hours until the solution no longer contains tiny particles, which can prevent agglomeration and speed up the dissolution; add 50-100g of polyvinylpyrrolidone to make it fully Dissolve; after cooling down to room temperature, add TMB0.4-1.0g, stir to fully di...

Embodiment 1

[0048] This embodiment provides a one-component TMB chromogenic solution, which is prepared by mixing liquid A and liquid B at a volume ratio of 1:1.

[0049] Described A liquid comprises each component of following concentration:

[0050] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8

[0051] Carbamide peroxide: 2mmol / L

[0052] Described B liquid comprises each component of following concentration:

[0053] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8

[0054] Polyvinyl alcohol: 0.4% (w / v)

[0055] Polyvinylpyrrolidone: 5% (w / v)

[0056] TMB: 0.04% (w / v)

[0057] Glycerin: 1% (v / v)

[0058] The citric acid-sodium citrate buffer contains 0.07mol / L citric acid and 0.03mol / L sodium citrate, and the pH is 3.5-3.8; the polyvinyl alcohol is polyvinyl alcohol 1788; the polyvinylpyrrolidone is polyvinyl alcohol Vinylpyrrolidone K-30.

[0059] The present invention also provides a preparation method for the above-mentioned one-component TMB chromogenic solu...

Embodiment 2

[0069] This embodiment provides a one-component TMB chromogenic solution, which is prepared by mixing liquid A and liquid B at a volume ratio of 1:1.

[0070] Described A liquid comprises each component of following concentration:

[0071] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8

[0072] Carbamide peroxide: 10mmol / L

[0073] Described B liquid comprises each component of following concentration:

[0074] Citric acid-sodium citrate buffer: 0.1mol / L, pH3.5-3.8

[0075] Polyvinyl alcohol: 0.7% (w / v)

[0076] Polyvinylpyrrolidone: 7.5% (w / v)

[0077] TMB: 0.07% (w / v)

[0078] Glycerin: 1.5% (v / v)

[0079] Raw material and preparation method are with embodiment 1.

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Abstract

The present invention provides a single-component TMB coloration liquid, which is formed by mixing a liquid A and a liquid B, wherein urea hydrogen peroxide is dissolved in a citric acid-sodium citrate buffer liquid to prepare the liquid A, and polyvinyl alcohol, polyvinyl pyrrolidone, TMB and glycerol are dissolved in a citric acid-sodium citrate buffer liquid to prepare the liquid B. Compared with the single-component TMB coloration liquid in the prior art, the single-component TMB coloration liquid of the present invention has the following advantages that: the stability of the coloration substrate in the solution can be maintained, the operator can be protect from being away from organic solvent hazard, the damage on the environment can be reduced, the stable aqueous solution system can be formed, and the single-component TMB coloration liquid can be stored for 24 months at a temperature of 2-8 DEG C.

Description

technical field [0001] The invention relates to a biological in vitro diagnostic reagent, in particular to a single-component TMB chromogenic solution and a preparation method thereof. Background technique [0002] Enzyme-linked immunoassay (ELISA) has the advantages of rapidity, sensitivity, simplicity, and easy standardization, and has become one of the mainstream technologies in the field of protein detection. Taking the ELISA indirect method as an example, its main working principle is: the antigen is coated on the microplate to form a solid phase carrier. If there is a corresponding IgG antibody in the sample, the antibody will combine with the antigen immobilized on the microtiter plate to form an immune complex of antigen-IgG antibody, and the remaining unbound components will be removed by washing. Add horseradish peroxidase (HRP)-labeled anti-human IgG antibody for the second step of incubation, combine with the above immune complex to form a new immune complex ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/543
CPCG01N33/54393
Inventor 吴恩应杨洋吴灼斌陈玉琴林桓旭刘爱和郭采平王锦才丁玉江黄伟荣张信
Owner SHENZHEN WEIGUANG BIOLOGICAL PROD