Synthetic phase shifting digital holographic microscopy

A technology of digital holographic microscopy and phase shifting, which is used in instruments and other directions

Inactive Publication Date: 2015-12-02
UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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  • Abstract
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Problems solved by technology

[0004] The purpose of the present invention is in order to overcome the deficiencies in the prior art, a kind of new phase-shifting algorithm

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  • Synthetic phase shifting digital holographic microscopy
  • Synthetic phase shifting digital holographic microscopy
  • Synthetic phase shifting digital holographic microscopy

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Embodiment Construction

[0062] In order to better explain the present invention, the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0063] Such as Figure 1a As shown, the traditional phase-shift digital holographic microscope system uses a microscope with high numerical aperture to amplify the object light field containing sample information and then interferes with the reference light. However, it is difficult to manufacture a high numerical aperture lens, and after using a microscope, the phase change characteristics of the lens will introduce additional phase coverage sample information; Figure 1b It is a synthetic phase shift digital holographic microrecording structure proposed by the present invention, and there is no microlens device in the system.

[0064] figure 2 It is a structural diagram of the optical path of the synthetic phase-shift digital holographic microscopy technology proposed by the present invention. The s...

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Abstract

The invention discloses synthetic phase shifting digital holographic microscopy. A large quantity of low-resolution phase shifting holograms is synthesized to form a high-resolution phase shifting hologram through ingenious design of a synthetic phase shifting algorithm for solving a linear system of equations, then the high-resolution phase shifting hologram is subjected to inverse transformation, and a complex amplitude image with high resolution and signal-to-noise ratio is reproduced. Compared with traditional phase shifting digital holography, the synthetic phase shifting digital holographic microscopy can improve the holographic imaging resolution remarkably; compared with synthetic aperture digital holographic microscopy, the synthetic phase shifting digital holographic microscopy has the advantage that the high-resolution hologram can be acquired accurately without a splicing fusion process of multiple holograms.

Description

technical field [0001] The invention relates to a digital holographic microscopic technique, in particular to a digital holographic microscopic technique using a synthetic phase shift algorithm. Background technique [0002] Digital holographic microscopy technology is a new type of microscopic imaging technology developed based on digital holography combined with optical microscopy technology. See (J. Opt. Soc. Am. 4(1), 159-165, 1987). However, the development of digital holography technology has encountered a huge bottleneck, that is, the resolution of CCD is low and the bandwidth is small. At present, most of the common CCDs on the market are megapixels, and the resolution is low, which is far from meeting the actual needs of digital holographic microscopy. In the lensless holographic optical path, the density of interference fringes is usually less than 1um×1um, so the existing CCD usually cannot record the complete information of interference fringes. [0003] In or...

Claims

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Application Information

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IPC IPC(8): G03H1/08
Inventor 史祎诗李拓许文慧张骏杨秀波罗勇
Owner UNIVERSITY OF CHINESE ACADEMY OF SCIENCES
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