Process for the extraction of polyhydroxyalkanoates from biomass

A technology of biomass and biomass particles, applied in solid solvent extraction, fermentation, etc.

Inactive Publication Date: 2015-12-02
VEOLIA WATER SOLUTIONS & TECH SUPPORT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Said need is not easy to meet because of the issues involved in the evaluation of PHA-containing biomass and adjusting batch-to-batch specific PHA recovery conditions to deliver a product of consistent molecular weight quality while maintaining the maximum possible PHA-enriched biomass loading into For good gel formation and overall maximum achievable process productivity and economy in batch production
No such industrial-scale facility exists today for the recovery of PHA from mixed cultures

Method used

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  • Process for the extraction of polyhydroxyalkanoates from biomass
  • Process for the extraction of polyhydroxyalkanoates from biomass
  • Process for the extraction of polyhydroxyalkanoates from biomass

Examples

Experimental program
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Effect test

Embodiment 8

[0113] EQ-5

[0114] where X n is the non-PHA component of the biomass loaded into the extraction process. Thus in a calibrated system specifying the extraction temperature and solvent (Example 8), the loading dependence versus the off-rate can be described. We found that the assessment of polymer off-rate during recovery is important to accommodate batch-to-batch variability in PHA-enriched biomass quality with batch-specific adjustments to the PHA process recovery operating parameters.

[0115] Based on more information on the molecular weight distribution of PHA-containing biomass (Example 5) and extraction time, biomass solvent loading limits can be defined with the expectation that the recovered polymer will remain above the specified lower product quality limit for molecular weight (Example 5). Example 9). The biomass loading constraints can be compared to the resulting PHA solvent loading to be used, as this relates to practical process constraints for product reco...

Embodiment 1

[0181] Embodiment 1—general analysis method and material

[0182] Thermogravimetric Analysis (TGA)

[0183] Between 2 and 10 mg of finely ground dry PHA-enriched biomass sample was weighed and heated from room temperature to 550°C under an inert nitrogen atmosphere. The sample temperature was raised to 105°C at a rate of 10°C and the weight was equilibrated at 105°C for 10 minutes. The samples were evaluated for moisture loss at 105°C. The temperature was again increased at a rate of 10°C until 550°C and weight loss was recorded. The ash content of the sample was evaluated from weight loss while maintaining the sample at 550° C. for 30 minutes in air. Polymers in both biomass and extracted PHA resins were quantitatively evaluated by this standard method. The weight loss and the change in the rate of weight loss with temperature were taken into account. These trends inform the thermal decomposition temperature (T d )PHAT d , the PHA content of the biomass and the pur...

Embodiment 2

[0202] Example 2 - Kinetics of PHA Dissolution

[0203] It is practical to measure the natural dissolution rate of poly(3-hydroxybutyrate) (PHB) in PHA-poor solvent as a benchmark. PHB is considered a "worst case scenario". Due to the tendency for higher crystallinity with PHB, it is considered to be more difficult to dissolve, especially in PHA-poor solvents. The dissolution trend of finely divided pure PHB powder (>98% purity) as a function of temperature was studied. Weigh PHB powder into a 12mL test tube and mix with 5mL equivalent 2-butanol to form a 50g-PHB / L solution. The tubes were sealed with Teflon-lined screw caps and introduced into a constant temperature heater at the selected temperature. The contents of the tube were vortexed every 3 minutes, and immediately after mixing, the solution optical density and color were measured. The relative change in solution color was used to indicate the progress of PHB dissolution in solution ( figure 1 , according to th...

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Abstract

An industrial scale batch or semi-continuous batch PHA extraction process wherein the process operating conditions and control may be quantitatively tuned to batch-to-batch variations based on a practical bench scale assessment but more preferably by means of a calibrated chemometric assessment of the biomass quality. Tuning involves the selection of biomass loading conditions that are commensurate with process solvent and PHA type. Batch operating times and temperatures are matched to modelled and monitored extraction kinetics. PHA-rich solvent gelation is exploited by controlling PHA-rich solvent gel formation. In combination a process Is disclosed that permits for robust and consistent recovered product quality control while at the same time enabling to improve overall process economics.

Description

technical field [0001] The present disclosure generally relates to the extraction and recovery of polyhydroxyalkanoates (PHAs) from biomass. Background of the invention [0002] Plastics today are mainly produced from petrochemical sources. However, growing concerns about the availability of natural resources and the accumulation of plastic waste in nature have stimulated increasing research and development focused on replacing petrochemical-based plastics with "bioplastics". If the plastic material is bio-based, biodegradable, or both, then the plastic material can be defined as bioplastic . [0003] Polyhydroxyalkanoates (PHAs) represent a class of bio-based polyesters that accumulate as intracellular particles in a variety of naturally occurring microorganisms. Methods for the production of PHA may include propagating pure bacterial or plant cultures dedicated to the production of PHA. PHA can also be recovered from open mixed cultures of biomass produced from biolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/89C12P7/62
CPCC08G63/89B01D11/02C12P7/625C08K3/014C08K5/0016C08K5/005C08G63/06
Inventor A.G.维尔克P.S.T.约翰松P.O.G.芒努松
Owner VEOLIA WATER SOLUTIONS & TECH SUPPORT
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