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Preparation method of caltrop saponin contained tribulus terrestris extract

A technology of Tribulus terrestris and extract, which is applied in the field of preparation of Tribulus terrestris extract, can solve problems such as difficulty in obtaining high-purity Tribulus terrestris saponins and high-efficiency acquisition of decomposing enzymes, and achieves excellent separation effect, low cost, and easy industrialization effect

Active Publication Date: 2015-12-09
内蒙古昶辉生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are various disadvantages in the above-mentioned methods, such as the difficulty in obtaining high-purity Tribulus terrestris saponins through ethanol aqueous extraction, the use of toxic solvents in methanol extraction after enzymolysis, and the complicated steps of obtaining decomposing enzymes in advance in the self-enzymatic hydrolysis method, and the decomposing enzymes are difficult to obtain. Efficiently obtain, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A preparation method of Tribulus terrestris extract, comprising the steps of:

[0035] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 8wt.% vinegar in the water, cook it and bury it for 2 hours, and let it cool naturally;

[0036] (2) Add 6 times ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;

[0037] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 1 times the column volume of water and 5 times the column volume of 20% ethanol solution to elute to remove impurities, and then eluted with 10 times the column volume of 50% ethanol solution, collect the eluate of 50% ethanol, recover the ethanol under reduced pressure and concentrate;

[0038] (4) Adjust the pH to about 5.0, use 5 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, re...

Embodiment 2

[0043] A preparation method of Tribulus terrestris extract, comprising the steps of:

[0044] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 5wt.% vinegar in the water, cook it and bury it for 2 hours, and let it cool naturally;

[0045] (2) Add 5 times of ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;

[0046] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 1 times the column volume of water, 5 times the column volume of 30% ethanol solution to elute to remove impurities, and then eluted with 10 times the column volume of 75% ethanol solution, collect the eluate of 75% ethanol, recover the ethanol under reduced pressure and concentrate;

[0047] (4) Adjust the pH to about 5.0, use 7 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, re...

Embodiment 3

[0052] A preparation method of Tribulus terrestris extract, comprising the steps of:

[0053] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 10wt.% vinegar in the water, cook it and bury it for 1 hour, and let it cool naturally;

[0054] (2) Add 8 times ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;

[0055] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 10 times column volume of water, 10 times column volume of 30% ethanol solution to elute to remove impurities, and then 10 times column volume of 70% ethanol solution to elute, collect the eluate of 70% ethanol, recover ethanol under reduced pressure and concentrate;

[0056] (4) Adjust the pH to about 4.0, use 8 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, recover tert-butyl met...

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Abstract

The invention provides a preparation method of tribulus terrestris extract. The preparation method includes the steps of performing raw materials mounding after water boiling via vinegar, extraction of ethyl acetate, column chromatography of polyamide and column chromatography of dry silica gel and microwave vacuum drying to finally obtain the tribulus terrestris extract 96wt.% greater than the caltrop saponin in content.

Description

technical field [0001] The invention relates to a preparation method of a tribulus terrestris extract, which belongs to the field of natural plant extracts. Background technique [0002] Tribulus terrestris (Tribulus terrestris L.) is a plant of the genus Tribulus terrestris in the family Tribulus terrestris. In recent years, with the deepening of research on Tribulus terrestris, flavonoids, alkaloids, organic acids, polysaccharides and steroidal saponins have been isolated from the plant, among which steroidal saponins have been studied more. [0003] Steroidal saponins are the main active ingredients of Tribulus terrestris. Studies have shown that Tribulus terrestris saponins can stimulate the secretion of luteinizing hormone in the human pituitary gland, promote the secretion of testosterone, increase the level of blood testosterone in the body, increase muscle strength, and promote physical recovery. It is used in training for bodybuilders and athletes of other competit...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P3/10A61P39/06A61P3/06A61P31/04A61P31/10A61P35/00A61P39/00A61P21/00
Inventor 马亚琼赵永强高瑞白莲婷贾洪涛
Owner 内蒙古昶辉生物科技股份有限公司
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