Preparation method of caltrop saponin contained tribulus terrestris extract
A technology of Tribulus terrestris and extract, which is applied in the field of preparation of Tribulus terrestris extract, can solve problems such as difficulty in obtaining high-purity Tribulus terrestris saponins and high-efficiency acquisition of decomposing enzymes, and achieves excellent separation effect, low cost, and easy industrialization effect
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Embodiment 1
[0034] A preparation method of Tribulus terrestris extract, comprising the steps of:
[0035] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 8wt.% vinegar in the water, cook it and bury it for 2 hours, and let it cool naturally;
[0036] (2) Add 6 times ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;
[0037] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 1 times the column volume of water and 5 times the column volume of 20% ethanol solution to elute to remove impurities, and then eluted with 10 times the column volume of 50% ethanol solution, collect the eluate of 50% ethanol, recover the ethanol under reduced pressure and concentrate;
[0038] (4) Adjust the pH to about 5.0, use 5 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, re...
Embodiment 2
[0043] A preparation method of Tribulus terrestris extract, comprising the steps of:
[0044] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 5wt.% vinegar in the water, cook it and bury it for 2 hours, and let it cool naturally;
[0045] (2) Add 5 times of ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;
[0046] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 1 times the column volume of water, 5 times the column volume of 30% ethanol solution to elute to remove impurities, and then eluted with 10 times the column volume of 75% ethanol solution, collect the eluate of 75% ethanol, recover the ethanol under reduced pressure and concentrate;
[0047] (4) Adjust the pH to about 5.0, use 7 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, re...
Embodiment 3
[0052] A preparation method of Tribulus terrestris extract, comprising the steps of:
[0053] (1) Take the raw material of Tribulus terrestris, cook it in water for 1 hour, add 10wt.% vinegar in the water, cook it and bury it for 1 hour, and let it cool naturally;
[0054] (2) Add 8 times ethyl acetate to the infiltrated raw material, extract for 2 hours, and filter to obtain the extract;
[0055] (3) Take the extract, adjust the pH to neutral, concentrate under reduced pressure, and separate it through a polyamide chromatography column. 10 times column volume of water, 10 times column volume of 30% ethanol solution to elute to remove impurities, and then 10 times column volume of 70% ethanol solution to elute, collect the eluate of 70% ethanol, recover ethanol under reduced pressure and concentrate;
[0056] (4) Adjust the pH to about 4.0, use 8 times the amount of tert-butyl methyl ether in the solution, extract in 3 times, separate the organic layer, recover tert-butyl met...
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