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Application of STAC2 gene and STAC2 gene coding protein in inhibition of pelvic organ prolapse

A pelvic prolapse and gene technology, applied in the field of biomedicine, can solve the problems of unknown etiology and pathogenesis, failure to fully clarify the mechanism of pelvic floor support tissue damage, and field limitations, and achieve simplified quantitative detection process, good application prospects, good stability effect

Active Publication Date: 2015-12-09
GUAN BOJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few basic studies on POP, and the fields involved are limited. Its etiology and pathogenesis are unknown, especially the basic research at the molecular level cannot fully clarify the damage mechanism of the pelvic floor support tissue, and more in-depth and extensive discussions are needed.

Method used

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  • Application of STAC2 gene and STAC2 gene coding protein in inhibition of pelvic organ prolapse
  • Application of STAC2 gene and STAC2 gene coding protein in inhibition of pelvic organ prolapse
  • Application of STAC2 gene and STAC2 gene coding protein in inhibition of pelvic organ prolapse

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The collection of embodiment 1 case

[0030] From September 2012 to December 2013, a total of 9 POP patients who visited the Department of Obstetrics and Gynecology of Union Medical College Hospital were collected. A total of 3 cases were collected using age and place of residence as matching factors. The uterosacral ligament tissue samples of all subjects were obtained, numbered and stored in a -80°C low-temperature refrigerator.

[0031] Inclusion criteria for the POP group: a. POP-Q score ≥ II degree after gynecological examination;

[0032] b. Continuously living in the Beijing area;

[0033] c. At least one childbirth history.

[0034] Exclusion criteria: a. Patients who received hormone replacement therapy in the past 3 months;

[0035] b. Patients with connective tissue diseases (rheumatoid arthritis, systemic erythema

[0036]lupus, polymyositis, Marfan syndrome, Eydown syndrome);

[0037] c. Pregnant patients;

[0038] d. The patient refuses to join;

...

Embodiment 2

[0051] Example 2 High-throughput sequencing and analysis

[0052] RNA extraction is performed on the tissue, followed by agarose gel electrophoresis after RNA extraction. From the results of electrophoresis, it can be preliminarily judged whether the quality of the extracted RNA sample is qualified or not, and whether it can be used for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0053] The sequencing platform is the HiSeq2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC (http: / / www.bioinformatics.babraham.ac.uk / projects / fastqc / ) software Overall assessment of the quality of sequencing data, including base quality value distribution, quality value position distribution, GC content, PCRduplication content, kmer frequency, etc. In t...

Embodiment 3

[0054] Example 3 The expression of STAC2 gene in the uterosacral ligament tissue of patients with pelvic cavity prolapse and controls

[0055] 1. Materials and methods

[0056] 1. Materials

[0057] The uterosacral ligament tissues of 35 cases of pelvic prolapse patients and 5 cases of control uterosacral ligament tissues were selected and grouped and numbered. The control group was patients with benign gynecological diseases undergoing laparotomy or vaginal hysterectomy, pelvic organ prolapse was ruled out by vaginal examination, and no urinary incontinence symptoms. Of the 35 patients with pelvic prolapse, 17 were grade II, 8 were grade III, and 10 were grade IV.

[0058] 2. Method

[0059] 2.1 Extraction of total RNA from uterosacral ligament tissues of patients with pelvic prolapse and controls

[0060] use Reagent (invitrogen, Cat. No. 15596-018) was used to extract sample RNA, and the experimental operation was carried out according to the product manual. The speci...

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Abstract

The invention relates to an application of a STAC2 gene and a STAC2 gene coding protein in inhibition of pelvic organ prolapsed. On the basis of high-throughput sequencing results, the candidate STAC2 gene in down-regulated expression is screened out. Further, according to experiments of cell biology, the STAC2 gene affects growth and proliferation activity of uterosacral ligament fibroblasts, is applicable to preparation of preparations for auxiliary diagnosis and treatment of pelvic organ prolapse and has significant clinical application values.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of STAC2 gene and its coded protein in inhibiting pelvic cavity prolapse. Background technique [0002] Pelvic organ prolapse (Pelvic organ prolapse, POP) is a complex multifactorial disease caused by environmental and genetic factors. In addition to environmental factors such as vaginal delivery, pregnancy injury, changes in hormone levels, aging, obesity, and long-term increased abdominal pressure, which can increase the susceptibility to POP, individual genetic factors also play an important role in the occurrence of POP. At present, it is believed that POP is a degenerative disease of pelvic floor muscles caused by relaxation of pelvic floor support tissue due to degeneration and injury. However, there are few basic studies on POP, and the fields involved are limited. Its etiology and pathogenesis are unknown, especially the basic research at the molecular level ha...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/577C12N15/11A61K48/00A61K38/00A61P15/00
CPCA61K38/00A61K48/00C12N15/11C12Q1/6883C12Q2600/158G01N33/577
Inventor 董琳杨承刚
Owner GUAN BOJIAN BIOTECH CO LTD
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