Immunoaffinity method

Abstract

The invention discloses an immunoaffinity method. The method comprises the following steps: filling an immunoaffinity column with a corresponding antibody matrix according to the concrete target of an analysis object; injecting an antibody active liquid to keep the activity of the antibody in order to make the highest liquid level be lower than the highest liquid level scale of the immunoaffinity column, screwing upper and lower end caps, and placing the obtained immunoaffinity column in appropriate ambient temperature; selecting single immunoaffinity column or combining a plurality of immunoaffinity columns according to the concentration range of the target, and determining the quantity of the immunoaffinity columns; and adding a target eluent, and collecting and analyzing an eluate flowing out of the immunoaffinity column(s). The immunoaffinity column(s) adopt(s) a telescopic spiral combination type immunoaffinity column. The immunoaffinity method improves the volume of the active liquid, enhances the integral sealing property of immunoaffinity, guarantees the sufficient antibody active liquid and prevents overflow and liquid leakage.

Description

technical field [0001] The present invention relates to an immunoaffinity method. Background technique [0002] As a sample pretreatment technology, solid phase extraction columns have been widely used in laboratories. With the continuous development of solid phase extraction technology, a new type of extraction column - immunoaffinity column is quietly replacing the traditional solid phase extraction column. Immunoaffinity column (IAC) is based on the antigen-antibody reaction, a separation and extraction column made of biomacromolecules with the characteristics of specific recognition and reversible binding to a class of biomacromolecules. It has unique selection specificity and good adsorption Purification performance. When the treated sample extract slowly passes through the immunoaffinity column, the analyte in the sample extract binds to the antibody in the column, while other impurities that are not bound are removed after washing, and then the column is washed with...

AI Technical Summary

Problems solved by technology

When the target exceeds the antigen limit that the antibody in the column can bind, the target will be lost, and the concentration of the target cannot be analyzed effectively

[0006] 3. In the process of using the immunoaffinity column, the turbidity of the analytical sample solution often causes the clogging of the affinity column, which affects the antibody-antigen binding ability of the affinity column, and even subsequent steps such as washing and elution cannot be carried out normally.

[0007] The above factors limit the popularization and commercialization of immunoaffinity columns, and a simple and effective device is needed to solve these problems

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