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Method for extracting microbial degradation hydrophobic organic compound from cloud point system

A technology of microbial degradation and cloud point system, which is applied in the field of extracting microbial degradation of hydrophobic organic matter, can solve the problems of unreported bioavailability, achieve the effect of ensuring continuous degradation and improving biodegradation efficiency

Active Publication Date: 2015-12-16
JIANGXI UNIV OF SCI & TECH
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  • Abstract
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Problems solved by technology

[0004] Although the cloud point system has been applied in biotransformation, as a new two-phase distribution system, the cloud point system has not been reported in the microbial degradation of hydrophobic organic matter and the bioavailability of pollutants.

Method used

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  • Method for extracting microbial degradation hydrophobic organic compound from cloud point system

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Embodiment 1

[0030] Under the condition that the non-ionic surfactant TritonX-114 was added in 4g / 100mL and phenanthrene was added in 40mg / 100mL, Sphingomonas was reacted in the formed cloud point system under the degradation conditions for 6 days. The result: phenanthrene The degradation rate is 69.07%.

Embodiment 2

[0032] Under the condition that the non-ionic surfactants TritonX-114 and TritonX-45 are compounded in a weight ratio of 8:2 and the addition amount is 4g / 100mL, and the addition amount of phenanthrene is 40mg / 100mL, the formation of Sphingomonas In the cloud point system, the reaction was carried out under the degradation conditions for 6 days. The result: the degradation rate of phenanthrene was 85.41%.

Embodiment 3

[0034] Under the condition that the addition amount of nonionic surfactant Brij30 is 4g / 100mL and the addition amount of phenanthrene is 40mg / 100mL, Sphingomonas reacts in the formed cloud point system under the degradation conditions for 6 days, the result: degradation of phenanthrene The rate is 57.68%.

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Abstract

The invention discloses a method for extracting a microbial degradation hydrophobic organic compound from a cloud point system and belongs to the technical field of environmental protection. The method comprises the following steps that A, a nonionic surfactant is adopted for forming the cloud point system at the temperature lower than the temperature of the microbial degradation hydrophobic organic compound; and B, the cloud point system is used as a medium of the microbial degradation hydrophobic organic compound, and the hydrophobic organic compound in the environment is extracted and solubilized into the degradation culture medium so as to degrade the hydrophobic organic compound. The method is applied to the aspect of degrading hydrophobic organic contaminants of the cloud point system, and no negative effects are caused to microbial survival and degradation vitality; and meanwhile, the bioavailability of the hydrophobic organic compound is improved, and environment pollution treatment is facilitated.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a method for extracting microorganisms to degrade hydrophobic organic matter in a cloud point system. Background technique [0002] Microbial degradation of hydrophobic organic compounds is often restricted by low substrate solubility, low bioavailability, and poor microbial growth environment. In view of this situation, the two-phase distribution system can be used to enhance the bioavailability of organic matter in the process of microbial degradation. The existing technology mainly includes the "liquid-liquid two-phase distribution system" composed of water-organic solvent and the composition of water-polymer "Liquid-solid two-phase distribution system". [0003] The cloud point system is a two-phase distribution system composed of an aqueous solution of nonionic surfactants, which is divided into two parts: a hydrophobic coacervate phase and a hy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D101/20
Inventor 潘涛曾新颖邓涛董伟余水静
Owner JIANGXI UNIV OF SCI & TECH
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