A method and system for increasing multiphoton imaging signal intensity

A multi-photon imaging and signal strength technology, applied in the field of biophotonics, can solve the problems of signal depletion and the inability to further improve the imaging depth, and achieve the effect of improving imaging depth and enhancing signal strength

Active Publication Date: 2017-10-20
SHENZHEN UNIV
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Problems solved by technology

[0005] In view of this, the purpose of the embodiments of the present invention is to provide a method and system for increasing the signal intensity of multiphoton imaging, which aims to solve the problem in the prior art that the imaging depth cannot be further improved due to signal depletion at a large imaging depth. question

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  • A method and system for increasing multiphoton imaging signal intensity
  • A method and system for increasing multiphoton imaging signal intensity
  • A method and system for increasing multiphoton imaging signal intensity

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Embodiment Construction

[0049] In order to make the objectives, technical solutions and advantages of the present invention clearer, the following further describes the present invention in detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

[0050] The specific embodiment of the present invention provides a method for increasing the signal intensity of multiphoton imaging, which mainly includes the following steps:

[0051] S11. Obtain the signal intensity of multiphoton imaging;

[0052] S12. In the objective lens, the signal intensity of the multiphoton imaging is enhanced by changing the filling factor of the objective lens.

[0053] The method provided by the present invention to improve the signal intensity of multiphoton imaging has been proved by numerical simulation in different l depth / l e It has the best laser beam fill fa...

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Abstract

The present invention is applicable to the field of biophotonics, and provides a method for increasing the signal intensity of multiphoton imaging, including: obtaining the signal intensity of multiphoton imaging; in the objective lens, enhancing the signal of the multiphoton imaging by changing the filling factor of the objective lens strength. The invention also provides a system for increasing the signal intensity of the multiphoton imaging. The invention can improve the maximum signal intensity in high-order multiphoton microscopic imaging of deep biological tissues, thereby obtaining greater imaging depth.

Description

Technical field [0001] The present invention relates to the field of biophotonics, in particular to a method and system for improving the intensity of multiphoton imaging signals. Background technique [0002] Multiphoton microscopy (ie multiphoton imaging) technology can perform non-invasive deep tissue imaging of living organisms, with sub-cellular resolution and three-dimensional imaging capabilities, and is mainly used in neurology, embryology, oncology and other fields Researchers can visualize the morphology and physiological structure of tissues in vivo or in vitro. [0003] Multiphoton imaging technology requires n (n is greater than or equal to 2, and n is an integer) photons to simultaneously excite the sample, and then emit a fluorescent photon signal. For example, two-photon microscopy imaging technology is one of the most commonly used multiphoton imaging techniques. Using this technology for uniformly labeled live mouse brain imaging, the deepest imaging depth is 1mm...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G02B21/36
Inventor 王科邱娉梁闰富
Owner SHENZHEN UNIV
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