Tissue culturing and rapid seedling raising method for Ludisia discolor (Ker-Gaw 1.)A.Rich seeds

A technology of tissue culture and blood leaf orchid, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inconsistent seed maturity, short fruit development cycle, difficult germination of blood leaf orchid, etc., and achieve simple equipment, The effect of improving the multiplication coefficient and seedling quality to meet the needs of industrialized large-scale production

Active Publication Date: 2015-12-23
福建省农业科学院农业生物资源研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] This application provides a method for tissue culture and rapid seedling cultivation of blood leaf orchid seeds, which solves the problems that blood leaf orchid is difficult to germinate in the natural

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of tissue culture of blood leaf orchid seed and the steps of rapid seedling raising method are as follows:

[0031] 1) Selection of culture materials: During the flowering stage of the blood leaf orchid, select the excellent strains that grow robustly for pollination of the same plant or different plants. After 33 days of pollination, pick the fruit that is white or light yellow in appearance and uncracked as the culture material for tissue culture;

[0032] 2) Induced culture of protocorms: comprising the following steps

[0033] ①Disinfection treatment: Soak the fruit selected in step 1 with 80% alcohol for 35 seconds, rinse it once with sterile water, and then carry out secondary disinfection; the secondary disinfection is to pour the fruit first Put it into a Tween 20 solution with a mass percentage concentration of 0.01%, shake it for 10 seconds, clamp out the fruit, and put it in a 10% mass percentage concentration of sodium hypochlorite solution for disinfe...

Embodiment 2

[0042] A kind of tissue culture of blood leaf orchid seed and the steps of rapid seedling raising method are as follows:

[0043] 1) Selection of culture materials: the fruit appearance color of wild blood leaf orchid plants collected in the field is white or nearly yellow and unbroken capsules are used as culture materials for tissue culture;

[0044] 2) Induced culture of protocorms: comprising the following steps

[0045] ① Disinfection treatment: soak the fruit selected in step 1 with alcohol with a mass percentage concentration of 90% for 50 seconds, rinse it with sterile water once, and then carry out secondary disinfection; the secondary disinfection is to place the fruit in Disinfect in the mixed solution of Tween 20 and sodium hypochlorite for 8-12 minutes and rinse with sterile water. In the mixed solution of Tween 20 and sodium hypochlorite, the mass percent concentration of Tween 20 is 0.01-0.02%, and the mass percent concentration of sodium hypochlorite 10-15%; ...

Embodiment 3

[0054] 1) Selection of culture materials: During the flowering period of the blood leaf orchid, select the excellent strains with strong growth for pollination of the same plant or different plants. After 25 days of pollination, pick the fruit whose appearance is white or light yellow and uncracked as the culture material for tissue culture;

[0055] 2) Induced culture of protocorms: comprising the following steps

[0056] ①Disinfection treatment: Soak the fruit selected in step 1 with 95% alcohol for 30 seconds, rinse it with sterile water for 2 times, and then carry out secondary disinfection; Put the Tween 20 solution with a mass percentage concentration of 0.02%, shake it for 20 seconds, clamp the fruit with tweezers, and place it in a 12% sodium hypochlorite solution for disinfection for 9 minutes;

[0057] ②Germination culture treatment: Rinse the disinfected fruit in step ① with sterile water, cut open the fruit with sterile dissecting tools, take out the white pulpy o...

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PUM

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Abstract

The invention relates to a tissue culturing and rapid seedling raising method for Ludisia discolor (Ker-Gaw 1.)A.Rich seeds. The method comprises the following steps: 1. culture material selection; 2, induction culture of protocorm; 3, multiplication culture of the protocorm; 4, differentiation and strong seedling culture. According to the method, the problems of germination difficulty of Ludisia discolor (Ker-Gaw 1.)A.Rich in a natural state as well as inconsistent seed maturity degrees, short service lives of the seeds and large amount of abortion caused by short developmental cycle of fruit are solved, and one method capable of rapidly reproducing Ludisia discolor (Ker-Gaw 1.)A.Rich seedlings is developed. The method has the advantages of convenient-to-obtain materials and high success rate and can be used for saving Ludisia discolor (Ker-Gaw 1.)A.Rich fruit with inconsistent seed development, so that large quantity of the Ludisia discolor (Ker-Gaw 1.)A.Rich seedlings can be produced, and the Ludisia discolor (Ker-Gaw 1.)A.Rich resources are better protected.

Description

technical field [0001] The application relates to a method for tissue culture and rapid seedling cultivation of blood orchid seeds, which is applied to the protection of blood orchid resources and the production of blood orchid seedlings. Background technique [0002] Blood leaf orchid Ludisia discolor (Ker-Gaw1.) A.Rich. is a plant of the genus Blood leaf orchid in the family Orchidaceae. There are 4 species in the genus, and there is only one species in my country, that is, blood leaf orchid. It is distributed on hillsides at an altitude of 900-1300 meters or in the damp places under evergreen broad-leaved forests in valleys. The rhizomes are prostrate, resembling silkworms, and the stem nodes take root. The leaves are purple-black with fine hairs, golden red or silvery white. The veins, like velvet, are very beautiful and have high ornamental value. At the same time, it is a folk traditional Chinese herbal medicine. The whole herb is used as medicine. It has the effects ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈菁瑛刘保财
Owner 福建省农业科学院农业生物资源研究所
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