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A high-efficiency extraction and separation process of anti-oxidative straw mushroom polysaccharides

An anti-oxidation and straw mushroom technology, applied in the direction of anti-virus, drug combination, etc., to achieve the effect of easy operation, simple process route, and less investment in equipment

Active Publication Date: 2017-04-26
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study on the antioxidant activity of straw mushroom polysaccharides has not been reported yet

Method used

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  • A high-efficiency extraction and separation process of anti-oxidative straw mushroom polysaccharides
  • A high-efficiency extraction and separation process of anti-oxidative straw mushroom polysaccharides
  • A high-efficiency extraction and separation process of anti-oxidative straw mushroom polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A high-efficiency extraction and separation process of anti-oxidation straw mushroom polysaccharide, the process comprises the following steps:

[0032] 1) Straw mushroom pretreatment: drying straw mushrooms to obtain dried straw mushrooms, and then degreasing to obtain defatted straw mushrooms;

[0033] 2) Pulverization: pulverize the defatted straw mushroom in step 1) to obtain straw mushroom powder, and the pulverization particle size is 40-80 mesh;

[0034] 3) Enzymatic extraction and enzyme inactivation: add 25 times the weight of water to the straw mushroom powder, then add 0.3% cellulase, keep the temperature of the material at 50°C, stir, and maintain the temperature for 120 minutes, then raise the temperature of the material to 90°C ℃, maintain 10min inactivation and then use the obtained material for later use;

[0035] 4) Centrifugation: cool the material in step 3) to 20°C, centrifuge at a speed of 3000r / min, and centrifuge for 10min, discard the precipitat...

Embodiment 2

[0044] A high-efficiency extraction and separation process of anti-oxidation straw mushroom polysaccharide, the process comprises the following steps:

[0045] 1) Straw mushroom pretreatment: drying straw mushrooms to obtain dried straw mushrooms, and then degreasing to obtain defatted straw mushrooms;

[0046] 2) Pulverization: pulverize the defatted straw mushroom in step 1) to obtain straw mushroom powder, and the pulverization particle size is 40-80 mesh;

[0047] 3) Enzymatic extraction and enzyme inactivation: add water 30 times the weight of straw mushrooms to the straw mushroom powder, then add 0.35% cellulase, keep the temperature of the material at 55°C, stir, and after maintaining the temperature for 90 minutes, raise the temperature of the material to 100 ℃, maintain for 5 minutes and inactivate the obtained material for later use;

[0048] 4) Centrifugation: Cool the material in step 3) to 40°C, centrifuge at a speed of 4000r / min, and centrifuge for 5min, discard...

Embodiment 3

[0053] A high-efficiency extraction and separation process of anti-oxidation straw mushroom polysaccharide, the process comprises the following steps:

[0054] 1) Straw mushroom pretreatment: drying straw mushrooms to obtain dried straw mushrooms, and then degreasing to obtain defatted straw mushrooms;

[0055] 2) Pulverization: pulverize the defatted straw mushroom in step 1) to obtain straw mushroom powder, and the pulverization particle size is 40-80 mesh;

[0056] 3) Enzymatic extraction and enzyme inactivation: add 27 times the weight of water to the straw mushroom powder, then add 0.33% cellulase, keep the temperature of the material at 53°C, stir, and maintain the temperature for 100 minutes, then raise the temperature of the material to 95 ℃, maintain for 8 minutes and inactivate the obtained material for later use;

[0057] 4) Centrifugation: Cool the material in step 3) to 30°C, centrifuge at a speed of 3500r / min, centrifuge for 7min, discard the precipitate, and ta...

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Abstract

The present invention discloses an efficient extraction and separation process of antioxidant mushroom polysaccharide. The process includes the steps of straw mushroom pretreatment, crushing, enzymatic extraction and enzyme inactivation, centrifugation, ultrafiltration separation, concentration and drying. Polysaccharide extraction by cellulase and multiple ultrafiltration are employed to obtain a polysaccharide obtained sample liquid. The enzymatic extraction process designed by the invention has high efficiency, and the mushroom polysaccharide has strong antioxidant activity. The yield of straw mushroom polysaccharide extraction by cellulase is 21.38%, which is 15.00% higher than the hot water extraction of polysaccharide. The ultrafiltration membrane separation process of straw mushroom polysaccharide designed by the present invention has the advantages of small equipment investment, simple process route and easiness to operate, and is applicable to mass production.

Description

technical field [0001] The invention belongs to the technical field of intensive processing of agricultural and sideline products, and in particular relates to a high-efficiency extraction and separation process of anti-oxidation straw mushroom polysaccharide. Background technique [0002] Straw mushroom is an important cultivated edible fungus in the world, and its total output ranks third among the four major cultivated edible fungi in the world. The annual output of straw mushroom in my country is more than 30,000 tons, accounting for 70% to 80% of the world's total output, ranking first in the world. the first. Straw mushrooms are the most difficult to keep fresh and store among edible fungi. The post-ripening effect after harvesting is very strong, and the temperature during the harvesting period is high, and they cannot be stored at low temperatures. These factors restrict the circulation of fresh straw mushrooms. and mass production. [0003] Free radicals in the hum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P39/06
Inventor 凡军民张雪松贾君谢春芹谢正林
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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