Method for determining content of methcathinone in sample by high performance liquid chromatography

A high-performance liquid chromatography and methcathinone technology, which is applied in the field of high-performance liquid chromatography to determine the content of methcathinone in samples, can solve the problems of reduced criminal investigation efficiency, low detection efficiency, and long detection time, and shorten Detection time, improved resolution, improved peak shape effects

Active Publication Date: 2015-12-23
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Application Information

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Problems solved by technology

Among them, in the process of using liquid chromatography to detect, there are still technical problems such as lon

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  • Method for determining content of methcathinone in sample by high performance liquid chromatography
  • Method for determining content of methcathinone in sample by high performance liquid chromatography
  • Method for determining content of methcathinone in sample by high performance liquid chromatography

Examples

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Example Embodiment

[0038] Example 1: Quantitative detection by internal standard method

[0039] The method for measuring the content of methcathinone in a sample by adopting high performance liquid chromatography in the present invention includes the following steps:

[0040] (1) Preparation of working solution: Take the standard stock solution of methcathinone and dilute it with a solution of the internal standard substance 2-phenylethylamine with a mass concentration of 0.1 mg / mL to produce methcathinone with a mass concentration of 0.5 and 0.1 respectively. , 0.05, 0.01, 0.005, 0.001, 0.0005mg / mL series of standard solutions, and maintain the concentration of the internal standard solution is 0.01mg / mL.

[0041] (2) Preparation of sample solution: Weigh about 45 mg of the sample material (the sample material of this example is prepared by mixing the methcathinone standard product with other substances, and the methcathinone content is 51.32wt%), Use a bottle-top pipette to add 20mL mobile phase ex...

Example Embodiment

[0047] Example 2: External standard method quantitative detection

[0048] (1) Preparation of working solution; take the standard stock solution of methcathinone and sequentially dilute it into a series of standard solutions with mass concentrations of 0.5, 0.1, 0.05, 0.01, 0.005, 0.001, 0.0005 mg / mL.

[0049] (2) Preparation of sample solution: Weigh about 45 mg of the sample material (the sample material of this example is prepared by mixing the methcathinone standard product with other substances, and the methcathinone content is 51.32wt%), Use a bottle-top pipette to add 20mL of mobile phase for extraction, shake for 10 minutes, centrifuge for 5 minutes, after centrifugation, use a 1mL pipette to absorb 1mL of supernatant, use a bottle-top pipette to add 10mL of mobile phase, shake evenly and take 1.5mL Install the automatic sample injection bottle to get the sample solution;

[0050] (3) Setting of detection conditions: adopt Agilent SB-Phenyl chromatographic column, specifica...

Example Embodiment

[0054] Example 3

[0055] The difference between this example and Example 1 lies in the sample and sample pretreatment process: the sample used is 45 mg of blood with a methcathinone content of 51.32wt%, that is, blank blood is taken and the methcathinone standard is added; Add 17 mL of a mixed solution of anisole, dichloromethane and diethyl ether to extract the methcathinone in the sample. The volume ratio of anisole, dichloromethane and diethyl ether is 5:2:1.5, and add to the separated organic phase Add 10 mL of hydrochloric acid with 15% HCl mass fraction, remove the organic phase, place the residue on a 35℃ rapid concentrator to concentrate to dryness, add 20 mL of mobile phase to extract with a bottle-top pipette, shake for 10 minutes, centrifuge for 5 minutes, and centrifuge Then use a 1mL pipette to suck up 1mL of the supernatant, add about 0.1mg of internal standard substance 2-phenethylamine, and use a bottle-top pipette to add 10mL of mobile phase. After shaking evenl...

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Abstract

The invention discloses a method for determining the content of methcathinone in a sample by high performance liquid chromatography. The method includes the steps of: (1) preparation of a working solution; (2) preparation of a sample solution; (3) setting of detection conditions; and (4) determination of the methcathinone content of the sample. The method provided by the invention adopts reversed-phase chromatography isocratic elution, can complete analysis within 12 minutes, has efficient and accurate detection result, can be used for determination of the methcathinone content of the sample, and provides technical support for conviction and sentencing of judicial departments.

Description

technical field [0001] The invention relates to the field of drug detection in the field of criminal investigation, in particular to a method for determining the content of methcathinone in a sample by using high performance liquid chromatography. Background technique [0002] Methcathinone is a new type of drug that has appeared in recent years and is quite harmful to the human body. This hazard not only affects the physiological state of the human body, but also affects people's psychological activities, and overdose can lead to death. The abuse of methcathinone is not only harmful to the human body, but also has adverse effects on the development of society. In the 2007 version of the "Catalogue of Narcotic Drugs and Psychotropic Substances" issued by the State Food and Drug Administration, the Ministry of Public Security, and the National Health and Family Planning Commission, methcathinone was listed as a class I psychotropic drug for control. [0003] The chemical na...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 常颖高利生郑珲张春水刘克林赵阳贺剑锋翟晚枫
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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