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Method for evaluating soil heavy metal pollution degree

A technology of pollution degree and evaluation method, applied in the field of genetic toxicology of environmental health, can solve problems such as ignorance of correlation

Inactive Publication Date: 2015-12-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to the current research, there is a good correlation between the micronucleus rate and chromosomal aberration rate of broad bean root tips and the toxicity of target pollutants such as alkaloids, antibiotics, and radiation, but the correlation between them and heavy metal pollutants is not yet known how

Method used

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  • Method for evaluating soil heavy metal pollution degree
  • Method for evaluating soil heavy metal pollution degree
  • Method for evaluating soil heavy metal pollution degree

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Embodiment Construction

[0031] In order to verify the correlation between the root tip micronucleus test of broad bean and the degree of heavy metal compound pollution, we designed an experiment.

[0032] 1. Sample collection

[0033] After on-the-spot investigation and research, and on the basis of comprehensive consideration of natural conditions such as landform, soil type, soil use, and various human factors in the research area, we formed a plan to arrange sampling points in Nanjing Industrial Zone. In April 2011, Sampling points are set radially from near to far, and at each sampling point, surface soil samples at a depth of 0-20cm are collected. During the collection process, each sample was precisely positioned by GPS, and the environmental conditions and geographical conditions near each sampling point were investigated. Remove plant residues, stones, etc. from soil mixture samples, and place them in a cool, ventilated place to dry naturally. See Table 1 for sampling point numbers, latitud...

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Abstract

The invention discloses a method for evaluating the soil heavy metal pollution degree. Songzi green broad beans are selected as biological indicators. The method comprises the steps of soil leachate extracting, seed steeping and germination accelerating, mutagenesis processing, root tip recovery culturing, fixing and storing, dissociating and dyeing, tabletting and microscopic examining, statistic analyzing, pollution index calculating and the like. According to the method, the eucaryon is adopted as the indicators for indicating the soil heavy metal pollution degree, the influence of soil heavy metal pollution on plants grown in land can be truly reflected, and the method for evaluating the soil heavy metal pollution degree is effective.

Description

technical field [0001] The invention relates to a method for evaluating the degree of heavy metal pollution in polluted soil, belonging to the field of environmental health genetic toxicology. Background technique [0002] The pedosphere is an important link connecting the atmosphere, hydrosphere, lithosphere, and biosphere. It is the base of human agricultural production and the foundation of terrestrial ecosystems. Through the material cycle and energy exchange with other layers, it affects the global changes in soil and the biological environment. Activity, diversity and productivity are the environmental elements most closely related to human beings. However, due to the rapid population growth and rapid industrial development, solid wastes are continuously piled and dumped on the soil surface, harmful waste water is continuously infiltrated into the soil, and harmful gases and floating dust in the atmosphere are also continuously falling into the soil with rainwater, res...

Claims

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Application Information

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IPC IPC(8): G01N33/24
Inventor 李梅刘凤崔益斌杨扬
Owner NANJING UNIV
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