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High-efficiency breeding method of purple potato tissue culture seedlings

A technology for purple potato and tissue culture seedlings, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, and can solve the problems of low cultivation efficiency of purple potato tissue culture seedlings, high incidence of virus in tissue culture seedlings, and cutting propagation speed of mini-potatoes. Slow and other problems, to achieve the effect of increasing the reproductive speed of test-tube seedlings, improving economic benefits and reducing reproductive costs

Active Publication Date: 2017-12-19
NINGBO ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problems of low cultivation efficiency of purple potato tissue culture seedlings, high incidence of virus in tissue culture seedlings, and slow propagation speed of micropotato cuttings in the prior art, the present invention provides a cultivation method with high cultivation efficiency, low incidence rate, good cutting effect and cultivation method. High-efficiency breeding method of low-cost purple potato tissue culture seedlings

Method used

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  • High-efficiency breeding method of purple potato tissue culture seedlings

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Effect test

Embodiment 1

[0044] 1) Ultra-low temperature treatment of purple potato tubers:

[0045]Take purple potato tubers with good appearance, healthy and disease-free, and exercise them at a low temperature of 0-2°C for 7 days, then perform ultra-low temperature treatment at -185°C for 0.5 hours, and then thaw and restore the purple potato tubers in the later stage.

[0046] 2) Induction culture of seed potato root tip and shoot tip:

[0047] Take the purple potato tubers with excellent appearance after recovery and culture and bury them in sterilized sand for cultivation. The sterilized sand environment is 15-17°C in temperature and 61-65% in humidity. After 5-8 weeks, it grows roots and new shoots. Induction culture of root tip and shoot tip;

[0048] Specifically include the following steps:

[0049] (1) Configure shoot tip induction medium: MS+0.09mg / L NAA+3.5% white sugar+70% soybean cake powder, pH5.8;

[0050] Cut off the terminal buds of 0.5-0.8cm new shoots, wash them with sterile wa...

Embodiment 2

[0070] 1) Ultra-low temperature treatment of purple potato tubers:

[0071] Take purple potato tubers with good appearance, healthy and disease-free, and exercise them at a low temperature of 0-2°C for 7 days, then perform ultra-low temperature treatment at -175°C for 0.5 hours, and then thaw and resume cultivation of purple potato tubers;

[0072] 2) Induction culture of seed potato root tip and shoot tip:

[0073] Take the purple potato tubers with excellent appearance after recovery and bury them in sterilized sand for cultivation. The temperature of sterilized sand is 15-17°C and the humidity is 61-65%. After 5-8 weeks, they will grow roots and new shoots. , inducing culture of root tip and shoot tip;

[0074] Specifically include the following steps:

[0075] Configure shoot tip induction medium: MS + 0.05mg / L NAA + 3.5% white sugar + 60% soybean cake powder, PH5.8; cut off the terminal buds of 0.5-0.8cm new shoots, first wash them with sterile water for 3-5 times, The...

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Abstract

The invention provides a high-efficiency breeding method of purple potato tissue culture seedlings. The high-efficiency breeding method of purple potato tissue culture seedlings comprises the following steps: ultra-low temperature treatment of purple potato tubers; seed potato root tip and shoot tip induction culture; strong seedling rooting culture, test tube plantlet virus detection; miniature tuber induction culture, miniature potato virus Detection; mini tuber transplanting and plug breeding. The high-efficiency breeding method of the purple potato tissue culture seedlings provided by the present invention adopts stem tip detoxification and root tip detoxification to cultivate miniature potato seedlings. The cultivation period of the root tip and the shoot tip is different, and the joint cultivation of the two can reduce the time cost. Effective reduction, fully and efficiently used seed potato resources, further reducing the propagation cost of tissue cultured seedlings, in addition, the propagation speed of test-tube seedlings cultivated by using root tips is fast, which improves work efficiency and reduces production costs.

Description

technical field [0001] The invention relates to plant tissue culture rapid propagation technology, in particular to a high-efficiency propagation method of purple potato tissue culture seedlings. Background technique [0002] Purple potato is an annual crop of Solanaceae, native to Australia. Purple potatoes, also known as black potatoes, are oblong in shape and have smaller bud eyes. The peel is dark purple, jet black and shiny, rich in luster. The flesh is dark purple, good-looking and attractive in color. The pulp starch content is as high as 13-15%, and the taste is good and the quality is excellent. According to the determination: every 100 grams of black potatoes contains 2.3 grams of protein, 0.1 grams of fat, 16.5 grams of carbohydrates, 11 milligrams of calcium, 1.2 milligrams of iron, 64 milligrams of phosphorus, 342 milligrams of potassium, 22.9 milligrams of magnesium, 0.01 milligrams of carotene, thiamine 0.1 mg riboflavin, 0.03 mg riboflavin, 16 mg niacin. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王芳黄坚刘健严成其陆旭峰
Owner NINGBO ACAD OF AGRI SCI