Application of Stearylethanolamine in Improving Botrytis Botrytis Resistance of Plants
A technology of stearoylethanolamine and plant ash, applied in the directions of application, plant growth regulator, plant growth regulator, etc., can solve the problems of less research and application, and achieve the advantages of extending the validity period, low cost, and improving the ability to resist rainwater erosion. Effect
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Embodiment 1
[0040] Get stearyl ethanolamine (SEA) 0.69g (purchased from SIGMA company), SEA is completely dissolved in the ethanol solution of 137.6mL, guarantees that no SEA is separated out; Slowly add the water of 154.9mL again, and mixed solution is mixed, finally Add 7.5mL of organosilicon solution to the mixed solution, stir evenly to obtain the preparation.
[0041] Get 100 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is five leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control. Randomly take tomato leaf samples sprayed with formulations and controls, use Trizol and SuperscriptII kits produced by Invitrogen, USA to extract, purify, and reverse-transcribe cDNA, and then use the Step ONE Plus Real-TimePCR System produced by Applied Biosystems, USA The instrument and t...
Embodiment 2
[0045] Weigh stearyl ethanolamine (SEA) 0.197g (SEA is purchased from SIGMA company), measure ethanol solution 39.3mL; SEA is completely dissolved in the ethanol solution, then slowly add 254.7mL of water, and the mixed solution is mixed evenly, Ensure that no SEA is precipitated; finally add 6.0 mL of organosilicon solution to the mixed solution, stir evenly to obtain the preparation.
[0046] Get 100 milliliters of the preparation of the present invention, add 10 liters of clear water and mix evenly, evenly spray on the surface of tomato blades whose seedling age is three leaves and one heart in the evening, until the leaves are wet, continue to spray for 2 days, use the tomato plants sprayed with clear water as control. The Botrytis cinerea pathogen was sprayed and inoculated on the plants treated with the preparation and the control, so that the suspension of the pathogen was evenly distributed on the leaves, and the inoculation concentration of the Botrytis cinerea pathog...
Embodiment 3
[0049] Get stearyl ethanolamine (SEA) 0.98g (purchased from SIGMA company), SEA is dissolved in the ethanol solution of 196.5% fully, guarantees that no SEA is separated out; Slowly add the water of 94.5mL again, and mixed solution is mixed, finally to Add 9.0 mL of organosilicon solution to the mixed solution, stir evenly to obtain the preparation.
[0050] Choose two adjacent plastic greenhouses with completely consistent cultivation technology and management, use the preparation of the present invention for the treatment shed in the rainy season at the end of spring, take 200 milliliters of the preparation of the present invention, add 20 liters of clear water and mix evenly, divide the preparation in the morning and evening Spray evenly twice on the surface of tomato leaves with a seedling age of seven leaves and one heart until the leaves are wet, and continue spraying for 2 days. The control booths were only sprayed with the same volume of water. Ten days later, the dis...
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