Application of Nitrogen Doped Carbon Dots in Analysis of Small Molecule Environmental Pollutants

A technology for nitrogen-doped carbon dots and environmental pollutants, which is applied in the analysis of materials, material analysis by electromagnetic means, and measurement devices, etc., can solve the problems of high toxicity, poor water solubility, complex synthesis process, etc. High and simple preparation steps

Inactive Publication Date: 2018-04-27
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these materials still have many deficiencies, such as poor water solubility, complex synthesis process, and high toxicity.

Method used

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  • Application of Nitrogen Doped Carbon Dots in Analysis of Small Molecule Environmental Pollutants
  • Application of Nitrogen Doped Carbon Dots in Analysis of Small Molecule Environmental Pollutants
  • Application of Nitrogen Doped Carbon Dots in Analysis of Small Molecule Environmental Pollutants

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Method for preparing nitrogen-doped carbon dots:

[0035] Step 1, weigh 0.2682g of malic acid in a microwave reactor, add 10mL of secondary water, then add 121μL of ethanolamine, stir well, and ultrasonically obtain a clear solution;

[0036] Step 2, the microwave reactor was placed in a microwave oven (700 watts) and reacted for 10 minutes under high heat to obtain a brown solid;

[0037] Step 3, take out the microwave reactor, cool it naturally, add 20mL of secondary water into it, stir and dissolve to obtain a brown solution, remove insoluble matter by filtration to obtain a clear brown solution, pass through a 500-1000Da dialysis bag, and dialyze in a glass container for at least Impurities were removed in 3 days, and a pure aqueous solution of nitrogen-doped carbon quantum dots was obtained.

Embodiment 2

[0039] Instrument and detection conditions: Bruker Autoflex II mass spectrometer (Bruker Daltonics, Germany); nitrogen laser, wavelength 337nm, negative ion mode; laser energy 60-70%; accumulation times: 500 times.

[0040] Solution configuration: the matrix is ​​made into a nitrogen-doped carbon dot aqueous solution with a concentration of 1.0mg / mL;

[0041] Pipette 1 μL of matrix solution onto the MALDI target, and after natural drying, MALDI-TOF MS analysis ( figure 1 ).

Embodiment 3

[0043] Instrument and detection conditions: Bruker Autoflex II mass spectrometer (Bruker Daltonics, Germany); nitrogen laser, wavelength 337nm, negative ion mode; laser energy 60-70%; accumulation times: 500 times.

[0044] Solution configuration: the matrix is ​​made into a carbon dot aqueous solution with a concentration of 2.0mg / mL; the mixture sample of 1-hydroxypyrene, 2,4-dihydroxyanthraquinone, and 11,12-dihydroxybenzopyrene is mixed with acetonitrile / acetone (1 : 1) is a solvent, and a solution with a concentration of 800 μg / mL is prepared.

[0045] Pipette 1 μL of the matrix solution on the MALDI target, and after natural drying, pipette 1 μL of the hydroxy polycyclic aromatic hydrocarbon mixture solution on the MALDI target, and after natural drying, perform MALDI-TOF MS analysis ( figure 2 ).

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Abstract

The invention discloses the application of nitrogen-doped carbon dots in the analysis of small molecular environmental pollutants, specifically a nitrogen-doped carbon dots (N-CDs) as a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS ) matrix to detect small molecule environmental pollutants. Using nitrogen-doped carbon dots as a substrate can efficiently and quickly realize the analysis and detection of small molecule environmental pollutants with m / z ranging from 150 to 1000. Suitable small molecule types include hydroxy polycyclic aromatic hydrocarbons, nitro polycyclic aromatic hydrocarbons, bisphenol compounds, perfluorinated acid environmental pollutants, etc. Using nitrogen-doped carbon dots as a matrix to detect small molecules can effectively eliminate the background interference of traditional organic matrix ion peaks, without adding ionizing agents, reducing the requirements for sample processing, and has good reproducibility and high salt tolerance It provides an efficient and rapid detection method for environmental pollutants, and expands the application range of carbon dots.

Description

technical field [0001] The invention relates to the use of nitrogen-doped carbon dots, in particular to the application of nitrogen-doped carbon dots as a MALDI-TOF MS matrix in the analysis of small molecule environmental pollutants. Background technique [0002] Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), since first reported by Karas et al., has become an effective tool for the analysis of non-volatile macromolecules such as proteins, peptides, carbohydrates, oligonucleotides Acids, oligosaccharides, synthetic polymers, etc. Its superior advantages include simple sample preparation, soft ionization, high sensitivity, good salt tolerance, fast and high efficiency. These features make MALDI-TOF MS more and more popular in sample detection and identification. Although it performs well in the analysis of high molecular weight compounds, due to the traditional organic matrices such as α-cyano-4-hydroxy-cinnamic acid (CHCA), 2,5...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/62
Inventor 路雯婧李勇董川双少敏蔡宗苇
Owner SHANXI UNIV
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