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A rapid screening and extraction method for unknown drug poisons in test materials

An extraction method and material inspection technology, which is applied in the field of chemical analysis and inspection, can solve the problems of one-time extraction of drug poisons, cumbersome operation, and heavy workload, so as to reduce the interference of impurities, increase the extraction rate, and achieve high extraction. rate effect

Active Publication Date: 2017-07-28
吴玉红
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects of cumbersome operation, heavy workload, long time-consuming, and inability to realize one-time extraction of all drugs and poisons existing in the extraction methods of common drugs and poisons in the test materials in the prior art, thereby providing A simple and fast method with high extraction rate, thorough impurity removal, and suitable for one-time extraction of all common drugs and poisons

Method used

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  • A rapid screening and extraction method for unknown drug poisons in test materials
  • A rapid screening and extraction method for unknown drug poisons in test materials
  • A rapid screening and extraction method for unknown drug poisons in test materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take two 0.5ml blood test materials, one of which is diluted with 0.5ml, pH=5.4 hexamethylenetetramine-hydrochloric acid buffer, mix well to obtain the first mixed solution, and transfer it to a container filled with 1.5g In the packed solid-phase extraction column, after the first mixed solution has completely infiltrated into the packing, take 7ml of ether for elution, and collect the ether eluate;

[0032] Another part of the test material was diluted with 0.5ml of potassium dihydrogen phosphate-sodium hydroxide buffer solution with pH=8, mixed well to obtain the second mixed solution, and transferred to another solid phase extraction column filled with 1.5g filler In the middle, after the second mixed liquid penetrates into the filler completely, take 7ml of dichloromethane for eluting, and collect the dichloromethane eluate;

[0033] The diethyl ether eluate and the dichloromethane eluate were respectively concentrated for inspection.

[0034] The filler in this emb...

Embodiment 2

[0036] Take two parts of 1ml of urine test material, one part is diluted with 1ml of citric acid-sodium citrate buffer solution with pH=5, mix well to obtain the first mixed solution, and transfer it to a solid-phase extraction machine filled with 2.3g filler In the column, after the first mixed solution has completely infiltrated into the filler, take 6ml of ether for elution, and collect the ether eluate;

[0037] Another part of the test material was diluted with 1ml of sodium carbonate-sodium bicarbonate buffer solution with pH=9.5, and mixed to obtain the second mixed solution, which was transferred to another solid-phase extraction column filled with 2.3g filler, and waited for After the second mixed solution is completely infiltrated into the filler, 6ml of dichloromethane is taken for elution, and the dichloromethane eluate is collected;

[0038] The dichloromethane eluate and ether eluate were concentrated for inspection respectively.

[0039] The filler in this embo...

Embodiment 3

[0041] Take two 0.5ml blood test materials, one of which is diluted with 0.5ml, pH=2 buffer solution, mix well to obtain the first mixed solution, and transfer it to a solid phase extraction column filled with 1.6g filler, After the first mixed solution has completely infiltrated into the filler, take 10ml of ether for elution, and collect the ether eluate;

[0042] Another part of the test material was diluted with 0.5ml of buffer solution with pH=14, mixed well to obtain the second mixed solution, and transferred to another solid-phase extraction column filled with 1.5g filler, and waited until the second mixed solution was completely After infiltrating into the filler, take 6.5ml of dichloromethane for eluting, and collect the dichloromethane eluate;

[0043] The diethyl ether eluate and the dichloromethane eluate were respectively concentrated for inspection.

[0044] The filler in this embodiment is composed of: based on the total mass of the filler, 1.32g of silicon dio...

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Abstract

The invention provides a rapid screening and extraction method of unknown medicines and toxins in samples. The method comprises the following steps: taking two samples; diluting one sample with a diluting solution with the pH of 2 to 14, and transferring the sample into a solid-phase extracting column; eluting with diethyl ether; diluting the other sample with the diluting solution with the pH of 2 to 14, and transferring the sample into the other solid-phase extracting column; eluting with dichloromethane; concentrating the eluents of the two times and testing, wherein the solid-phase extracting columns are filled with fillers with specific components and specific performances. According to the extraction method provided by the invention, all the medicines and toxins in the samples can be separated and extracted in one step within 5 minutes; the rapid screening and extraction method has the advantages of strong universality, simplicity and convenience in operation, small working amount, strong impurity removing capability and high extraction efficiency; the extraction rates of all common medicines and toxins are basically more than 90%.

Description

technical field [0001] The invention relates to the technical field of analysis and testing of chemicals, in particular to a rapid screening and extraction method for unknown drug poisons in test materials. Background technique [0002] The analysis and testing of drugs and poisons in the test materials is divided into two links: extraction and detection. Due to the rapid development of science and technology, instrumental analysis and detection technology has made great progress, but the extraction process is still time-consuming, cumbersome, low extraction rate, and impossible. Screening unknowns is currently the bottleneck restricting the development of drug poison analysis technology. Therefore, it is particularly important to establish a method that is simple and fast, has high extraction rate, complete removal of impurities, and is suitable for simultaneous extraction of multiple drugs and poisons. [0003] Usually, the most widely used extraction method is the classic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/50G01N30/88G01N21/33
Inventor 吴玉红
Owner 吴玉红