Method for rapidly detecting residual forchlorfenuron in cucumbers

Abstract

The invention discloses a method for rapidly detecting residual forchlorfenuron in cucumbers. The method comprises the first step of instrument and reagent selection, the second step of plant sample extraction, the third step of soil sample selection, the fourth step of purification and the fifth step of gas chromatography detection, wherein instruments adopt an electron capture detector and a full-automatic accelerated solvent extraction instrument, and reagents adopt acetone, dichloromethane, anhydrous sodium sulfate, sodium chloride, ethyl acetate, n-hexane and diatomaceous earth. According to the method for rapidly detecting the residual forchlorfenuron in the cucumbers, a gas chromatography detection method of a plant growth regulator forchlorfenuron in the cucumbers and soil is established, and the method has the advantages of being simple and convenient in pretreatment step, wide in use of instruments, high in recovery rate and low in minimum detection limit.

Description

technical field [0001] The invention relates to the technical field of detection and application of agricultural residues, in particular to a method for rapidly detecting residues of forchlorfenuron in cucumbers. Background technique [0002] Forchlorfenuron (forchlorfenuron), chemical name l-(2-chloro-4-pyridine)-3'-phenylurea. It is a phenylurea plant growth regulator with cytokinin activity, its biological activity is 10-100 times higher than that of 6-benzylaminopurine, and it is widely used in agriculture, horticulture and fruit trees [1-3]. With the emergence of Fujiminori grapes, large fruit has become a hot spot pursued by consumers. In order to cater to the psychology of consumers and obtain larger fruits, bulking agents are widely used in the cultivation of fruit trees. Existing report adopts high performance liquid chromatography to measure the residue of forchlorfenuron in cucumber and kiwi fruit, on the research of forchlorfenuron residue analysis method in gr...

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Problems solved by technology

Existing report adopts high performance liquid chromatography to measure the residue of forchlorfenuron in cucumber and kiwi fruit, on the research of forchlorfenuron residue analysis method in grape, DebiSharma and MaheshD.Awasthi adopt liquid-liquid extraction-high performance liquid chromatography, This method uses a large amount of organic reagents, and is prone to emulsification during the extraction process, and the purification steps are relatively cumbersome

Kaushik Banerjee used high-performance liquid chromatography-mass spectrometry to detect pesticide residues in grapes. The anion exchanger propylethylenediamine (PSA) was used to purify the matrix in pretreatment, but compared with solid-phase extraction column purification, the purification effect Not ideal, and this method has high requirements for equipment; it is not easy to promote and use in production