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Primer system and kit for detecting FLT3 (Fms-like tyrosine kinase 3) gene polymorphism

A gene polymorphism, detection kit technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. , Wide range of application fields, fast and convenient detection effect

Inactive Publication Date: 2016-01-13
上海赛安生物医药科技股份有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0003] FLT3ITD is an internal tandem repeat mutation of the FLT3 gene, which can be effectively detected by denaturing high performance liquid chromatography, capillary electrophoresis, direct sequencing, denaturing polyacrylamide electrophoresis, and high-resolution melting curves; however, when it involves the detection of multiple point mutations in FLT3, According to the routine, probe detection is generally used, which is time-consuming, laborious and expensive, and there is currently no effective and economical detection product

Method used

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  • Primer system and kit for detecting FLT3 (Fms-like tyrosine kinase 3) gene polymorphism
  • Primer system and kit for detecting FLT3 (Fms-like tyrosine kinase 3) gene polymorphism
  • Primer system and kit for detecting FLT3 (Fms-like tyrosine kinase 3) gene polymorphism

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Embodiment 1

[0028] 1. The composition of the kit.

[0029] The FLT3 gene polymorphism detection kit of this embodiment includes: a reaction solution A for detecting the case where the c.2503 site of the FLT3 gene is G, and a reaction solution B for detecting the case where the c.2503 site of the FLT3 gene is C, Reaction solution C for detection of FLT3 gene c.2504 site as A, reaction solution D for detection of FLT3 gene c.2504 site as C, and reaction solution D for detection of FLT3 gene c.2505 site as T Reaction solution E, for detection of FLT3 gene c.2505 site A reaction solution F, for detection of FLT3 gene c.2506 site A reaction solution G, for detection of FLT3 gene c.2506 site Reaction solution H for the case of T, and reaction solution I for detecting the β-actin gene as an internal control.

[0030] The preparation of reaction solution A, B, C, D, E, F, G, I is as follows:

[0031] 1. Preparation of reaction solution A (MIXA).

[0032] Reaction solution A is the reaction mix...

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Abstract

The invention relates to a primer for detecting FLT3 (Fms-like tyrosine kinase 3) gene polymorphism. The primer comprises a forward primer specific to a c.2503 site of FLT3 gene being a case G, a forward primer specific to the c.2503 site of the FLT3 gene being a case C, a forward primer specific to the c.2503 site of the FLT3 gene being a case A, a forward primer specific to a c.2504 site of the FLT3 gene being a case C, a forward primer specific to a c.2505 site of the FLT3 gene being a case A, a forward primer specific to a c.2506 site of the FLT3 gene being a case A, a forward primer specific to the c.2506 site of the FLT3 gene being a case T, a universal reverse primer specific to the c.2503, c.2504, c.2505 sites, a forward primer for detecting beta-actin gene, and a reverse primer for detecting the beta-actin gene. A kit for detecting FLT3 gene polymorphism allows quick and convenient detection, is high in sensitivity and accuracy and is low in cost.

Description

technical field [0001] The invention relates to a gene mutation detection product, a detection primer and a detection system used in the product, and belongs to the field of biotechnology. Background technique [0002] The FLT3 gene is FMS-like tyrosine kinase 3 (Fms-liketyrosinekinase 3), also known as fetal liver kinase 2 (fetalliverkinase-2) or human stem cell kinase 1 (humanstemcellkinase-1, STK-1), and colony-stimulating factor 1 receptor ( CSF1R or FMS), platelet-derived growth factor (PDGFR), stem cell factor receptor (KIT) belong to type Ⅲ receptor tyrosine kinase (class Ⅲ receptor tyrosine kinase, RTK Ⅲ) family. In recent years, studies have found that FLT3 gene mutation is closely related to the occurrence and prognosis of acute myeloid leukemia (AML), and the detection of FLT3 has become an important indicator for the diagnosis and prognosis of leukemia. Studies have shown that the frequency of FLT3 mutations in AML reaches 24.3%, mainly including FLT3ITD and FLT...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/118C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 赵新泰王明唐娟娟江雁翔
Owner 上海赛安生物医药科技股份有限公司
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