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One-step seedling culturing tissue culture and rapid propagation method of pennisetum hydridum

A technology for tissue culture, rapid propagation and giant Juncao, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high transportation cost, destruction of seedlings, and low utilization rate of seedlings

Active Publication Date: 2016-01-20
运城市绿碧源农林开发有限公司
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Problems solved by technology

The obvious disadvantage of this method is that the transportation cost is high, and the seedlings may be irreversibly damaged during transportation, resulting in low seedling utilization.

Method used

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Embodiment Construction

[0012] 1. Obtaining explants: Select Oasis No. 1 Juncao plants that grow vigorously and are free from diseases and insect pests in sunny days, cut off the stems as explants, cut off the leaves, peel off the leaf sheaths, and cut them into 2cm with alcohol-sterilized pruning shears For the left and right sections, each section has a node (axillary bud), 0.5 cm below the node and 1.5 cm above the node. For the stem section with nodes and axillary buds, without peeling off the axillary buds, the nutrition in the stem section can promote the rapid growth of the axillary buds.

[0013] 2. Disinfection of explants: 75% alcohol treatment for 30 seconds, then rinse with 80,000 units of gentamycin sterile solution on the ultra-clean workbench for 3 times, and disinfect with 1.5-2‰ of mercuric chloride for 5 -10 minutes and shake several times, and finally rinse with 80,000 units of gentamicin sterile solution for 3 times;

[0014] 3. Culture medium preparation: After all the medicines...

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Abstract

The invention relates to a tissue culture and rapid propagation culture medium of Lvzhou No.1 grass. A culture medium formula is formed by combining a culture solution with a low salt concentration with a plant growth conditioning agent; by using the tissue culture and rapid propagation culture medium provided by the invention, an optimal plant axillary bud stem section is used as an explant; in a whole sterile culture process, only one culture medium is needed; the culture process is simple and feasible. In the culture medium, subculture and rooting induction are carried out synchronously and one-step seedling culture is realized. A seedling culturing period of Lvzhou No.1 grass tissue culture seedlings is shortened and industrial production is facilitated. The invention further relates to a one-step seedling culturing tissue culture and rapid propagation method of the Lvzhou No.1 grass; a multiplication culture process is a manner of multiplying the seedlings after the seedlings obtained by culturing stem section buds (with bud sections) in one step are chopped, and the multiplication coefficient is 3 to 5; no variation phenomenon is caused and no callus is formed in a rooting process; base parts of axillary buds are used for rooting and the transplanting survival rate is high.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation medium and a tissue culture rapid propagation method of plants, in particular to a tissue culture rapid propagation medium and a tissue culture rapid propagation method of giant jungales. Background technique [0002] The new technology of Juncao was invented by Lin Zhanxi, a researcher at Fujian Agriculture and Forestry University and known as the "father of Juncao in the world". This technology has successfully solved the worldwide problem of "bacteria-forest contradiction", replacing wood with grass, turning waste into treasure, and making great contributions to solving human poverty and protecting the "lungs" of human beings - the forest. After nearly 30 years of innovative research and development, the technology has become increasingly mature. Nearly 60 species of weeds that can replace wood bacteria have been selected from thousands of weeds, and the research and application of Juncao h...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王晓翔吴慎杰李静张换样焦改丽
Owner 运城市绿碧源农林开发有限公司
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