A kind of tissue engineered cartilage culture device in vitro

Abstract

The invention discloses a tissue engineered cartilage in-vitro culture device. The tissue engineered cartilage in-vitro culture device comprises a machine frame. A cartilage culture mechanism is arranged on the machine frame. The tissue engineered cartilage in-vitro culture device further comprises a culture solution circulation mechanism used for conveying a culture solution to the cartilage culture mechanism, a dynamic static pressure exertion mechanism used for exerting dynamic static pressure on the cartilage culture mechanism, and a dynamic sealing mechanism used for controlling culture solution input and output of the culture solution circulation mechanism. By means of the tissue engineered cartilage in-vitro culture device, the static pressure frequency and magnitude of cartilage culture and the flowing situation of the culture solution are regulated, the environment of dynamic static pressure is simulated, and cartilage which is good in cell viability and good in cartilage form and has certain mechanical strength can be cultured.

Description

technical field [0001] The invention belongs to the technical field of biomedical engineering, and in particular relates to an in vitro culture device for tissue engineered cartilage. Background technique [0002] Cartilage plays an important role in the movement function of joints. However, articular cartilage damage is a very common disease. About 60% of patients undergoing arthroscopic surgery for various reasons have cartilage damage, and 20% of them have severe cartilage damage. Due to the lack of vascular tissue in cartilage and the fact that chondrocytes are wrapped in matrix components and cannot migrate to the damaged site to repair the defect, the self-healing ability of articular cartilage after injury is very poor and regeneration cannot be achieved. If cartilage damage is not treated in time, it will easily lead to softening of the articular surface in the later stage, and in severe cases, large pieces may fall off, exposing the subchondral bone layer, causing ...

AI Technical Summary

Problems solved by technology

[0004] However, when tissue engineering methods combine chondrocytes and biodegradable materials to construct tissue-engineered cartilage, static culture is usually used, which leads to the formation of defects such as hollows in the cartilage tissue constructed in vitro, and its mechanical properties are far from normal cartilage. Cannot meet the requirements of clinical transplantation

The methods of applying force involved in the dynamic culture of chondrocytes currently reported are mainly perfusion and direct application of pressure. The environment is not in a closed environment, and there are many defects such as limited culture time.

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