MS-free tissue culture method for bananas

A tissue culture and rooting tissue culture technology, applied in the agricultural field, can solve the problems of cumbersome steps, easy dependence, and expensive hormones, and achieve the effects of reducing production costs, rooting quantity and thickening effect, and ecological environment.

Active Publication Date: 2016-02-03
GUANGXI UNIV +1
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used substratum is MS (MurashigeandSkoog) medium, and it needs to add macronutrient, trace element organic matter, iron salt, sucrose, hormone, agar and distilled water when p

Method used

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  • MS-free tissue culture method for bananas

Examples

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Effect test

Embodiment 1

[0034] The following examples carry out tissue culture on the stages of multiplication, subculture and rooting culture of banana clusters.

[0035] (1) 30% CAAL medium bud induction culture of banana without MS

[0036] Take tissue-cultured bottle seedlings (banana adventitious buds), aseptically cut a single bud on an ultra-clean workbench, remove the leaves and browned parts, keep the growth point of no more than 1cm at the base, and transfer it to 30% cassava alcohol with or without MS In the medium of anaerobic liquid medium, 200g / l potato juice, 3.0mg / l6-BA and 0.2mg / lNAA, culture for 30-40 days, 26-28°C, light intensity 2000lx, light time 16h / d; culture medium The pH value is 5.6-5.8, and it contains 30g / l sucrose and 6.5g / l agar. The potato juice is mashed fresh potatoes, filtered to remove slag, and the juice is retained.

[0037] The 30% cassava alcohol anaerobic liquid (CAAL) medium without MS described in this example refers to the cassava alcohol anaerobic fermen...

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Abstract

The invention provides an MS-free tissue culture method for bananas. Clump shoot regeneration tissue culture liquid, subculture tissue culture liquid and root taking tissue culture liquid are adopted in the clump shoot regeneration stage, the subculture stage and the root taking culture stage of bananas respectively, and the lump shoot regeneration tissue culture liquid, the subculture tissue culture liquid and the root taking tissue culture liquid all contain a cassava alcohol anaerobic fermentation liquid culture medium. The technical scheme is adopted, cassava alcohol anaerobic fermentation liquid is used for replacing a basic culture medium MS, normal growth in three different stages of banana tissue culture is not affected at all, production cost is greatly reduced, waste is turned into valuable materials, the method is more environmentally friendly in the aspect of ecological environment, cultured banana seedlings can achieve the effect of seedlings which are cultured through MS tissue culture in the prior art, the root taking amount is larger, roots are thicker and stronger, the period of seedling establishment is earlier than that of the seedlings cultured through the basic MS culture medium, transplantation and survival of tissue culture seedlings are easier, and favorable conditions are provided for large-scale tissue culture production.

Description

technical field [0001] The invention belongs to the technical field of agriculture and relates to a method for tissue culture of bananas without MS. Background technique [0002] Banana (Musaparadisiaca L.) belongs to Musaceae (Musaceae) and the genus Musa (MusaL). It is a perennial large evergreen herbaceous monocotyledonous plant, which originated in tropical Asia and is one of the main fresh fruit species in the world. my country has been cultivating bananas for more than two thousand years, and is one of the countries with the longest history of cultivating bananas. South China is the main production area of ​​bananas in my country, and it is also one of the origins of bananas. [0003] At present, almost all of the commercially valuable banana cultivars in my country are vegetatively fruity, with no seeds in the fruit or completely degraded seeds, belonging to triploids (including AAA genotype, AAB genotype and ABB genotype), with a high degree of male and female Due to...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 邓英毅许娟郑虚韦民政李韦柳莫云川李峰潘介春莫干辉叶亦心孙宁静
Owner GUANGXI UNIV
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